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بررسی تاثیر اسانس برخی از کموتیپهای آویشن شیرازی و مورتلخ بر کنترل قارچ Aspergillus niger | ||
زیست شناسی کاربردی | ||
مقاله 6، دوره 37، شماره 4 - شماره پیاپی 82، دی 1403 | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22051/jab.2024.48402.1648 | ||
نویسندگان | ||
زینب مرادزاده1؛ سمیه رستگار* 2؛ علیرضا یاوری2 | ||
1دانش آموخته کارشناسی ارشد، گروه باغبانی دانشکده کشاورزی و منابع طبیعی دانشگاه هرمزگان، بندرعباس، ایران. | ||
2دانشیار گروه باغبانی دانشکده کشاورزی و منابع طبیعی دانشگاه هرمزگان، بندرعباس، ایران. | ||
چکیده | ||
مقدمه: در سالهای اخیر استفاده از متابولیتهای ثانویه برخی گیاهان دارویی در جلوگیری از رشد قارچها مورد توجه قرار گرفته است. هدف از این پژوهش بررسی اثرات ضد قارچی دو اسانس آویشن شیرازی و مورتلخ با دو روش کشت تدخینی و ترکیبی (اختلاط اسانس با محیط کشت) در کنترل قارچ آسپرژیلوس نایجر در شرایط آزمایشگاهی بود. روشها: در این پژوهش طی دو آزمایش جداگانه تاثیرکموتیپهای مختلف اسانس آویشنشیرازی با غلظتهای 50، 100، 200، 400 میکرولیتر در لیتر و مورتلخ با غلظتهای 100، 200، 400 میکرولیتر در لیتر، بر کنترل رشد قارچهای آسپرژیلوس به دو روش کشت قارچ (تدخینی و ترکیبی) در شرایط آزمایشگاهی مورد ارزیابی قرار گرفت. نتایج و بحث: براساس نتایج آزمایش اول، در بررسی جلوگیری از رشد قارچ آسپرژیلوس، کموتیپ نصرآباد اصفهان در یک گروه آماری، کموتیپهای بوشهر و طشک فارس در یک گروه آماری و سایر کموتیپها در گروه آماری دیگر قرار گرفتند. تفاوت معنی-داری بین روشهای مختلف کشت قارچ مشاهده نشد. با افزایش غلظتهای اسانس تاثیر آن درجلوگیری از رشد هاله افزایش یافت. در آزمایش دوم، در غلظت 400 میکرولیتر در لیتر، بیشترین تاثیر اسانس مورتلخ در جلوگیری از رشد قارچ آسپرژیلوس در کموتیپ بستک هرمزگان مشاهده شد که نسبت به سایر کموتیپهای مورتلخ تفاوت معنیداری نشان داد. در این آزمایش نیز تفاوت معنیداری بین دو روش کشت قارچ مشاهده نشد. بطور کلی اسانسهای استفاده شده نقش موثری در کنترل رشد قارچ آسپرژیلوس نشان دادند البته تفاوت معنیداری بین کموتیپهای مختلف دو اسانس آویشنشیرازی و مورتلخ مشاهده شد. | ||
کلیدواژهها | ||
اسانس؛ آویشن؛ درون شیشه؛ قارچ؛ مورتلخ | ||
عنوان مقاله [English] | ||
Evaluation of the effect of essential oil of some chemotypes of Zataria multiflora and Salvia mirzayanii on the control of Aspergillus niger | ||
نویسندگان [English] | ||
Zeinab Moradzadeh1؛ Somayeh Rastegar2؛ Alireza Yavari2 | ||
1Master's degree student, Department of Horticulture, Faculty of Agriculture and Natural Resources, University of Hormozgan, Bandar Abbas, Iran. | ||
2Associate Professor, Department of Horticulture, Faculty of Agriculture and Natural Resources, University of Hormozgan, Bandar Abbas, Iran. | ||
چکیده [English] | ||
Introduction: The purpose of this study was to investigate the antifungal effects of two essential oils of Zataria multiflora and Salvia mirzayanii with two methods of smoking and mixed culture (mixing essential oil with culture medium) in controlling Aspergillus niger fungus in laboratory conditions. Methods: In this research, during two separate experiments, the effect of different chemotypes of Zataria multiflora essential oil with concentrations of 0, 50, 100, 200, 400 microliters per liter and Salvia mirzayanii with concentrations of 100, 200, 400 microliters per liter, on growth control Aspergillus fungi were evaluated by two cultivation methods (smoked and mixed) in laboratory conditions. Result and discussions: Based on the results of the first experiment, in the investigation of preventing the growth of AJspergillus fungus, the Isfahan Nasrabad chemotype was placed in one statistical group, the Bushehr and Tashk Fars chemotypes were in one statistical group, and the other chemotypes were in another statistical group. No significant difference was observed between different mushroom cultivation methods. By increasing the concentration of essential oil, its effect in preventing halo growth increased. In the second experiment, at a concentration of 400 microliters per liter, the greatest effect of bitter gourd essential oil in preventing the growth of Aspergillus fungus was observed in Hormozgan Bastak chemotype, which showed a significant difference compared to other gourd chemotypes. In this experiment, no significant difference was observed between the two mushroom cultivation methods. | ||
کلیدواژهها [English] | ||
Essential oil, Thyme, Fungus, Salvia | ||
مراجع | ||
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