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جداسازی و شناسایی مولکولی باکتری‌های محرک رشد گیاه از فیلوسفر و ریزوسفر گندم زراعی (Triticum aestivum L. ) | ||
| زیست شناسی کاربردی | ||
| مقاله 4، دوره 37، شماره 3 - شماره پیاپی 81، آذر 1403، صفحه 36-46 اصل مقاله (931.01 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2024.44085.1574 | ||
| نویسندگان | ||
| فرحناز جوانمردی* 1؛ سید محمد مهدی محمودی2؛ حسن رستمی3 | ||
| 1استادیار، گروه زیست شناسی، دانشکده علوم پایه، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران | ||
| 2استادیار ،گروه میکروبیولوژی ، دانشکده علوم پایه، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران | ||
| 3دانش آموخته کارشناسی ارشد، گروه میکروبیولوژی، دانشکده علوم پایه، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران | ||
| چکیده | ||
| مقدمه: تعداد قابل توجهی از باکتری‌های خاک دارای قابلیت تثبیت نیتروژن، محلول‌سازی فسفات و تولید اکسین هستند. چنین باکتری‌هایی می‌توانند نقش موثری در بهبود رشد گیاهان داشته باشند. هدف از این پژوهش، جداسازی، شناسایی و بررسی عملکرد چنین باکتری هایی بر میزان رشد گیاه عدس بود. مواد و روشها: . تعداد 30 نمونه باکتری، از فیلوسفر و ریزوسفر گندم جداسازی شدند. از محیط کشت فاقد نیتروژن جهت شناسایی باکتری‌های تثبیت کننده نیتروژن، و از محیط کشتNBRIP جهت بررسی توانایی محلول‌سازی فسفات نامحلول استفاده شد. توانایی جدایه‌ها در تولید اکسین با روش سالکوفسکی بررسی شد. همچنین تاثیر دو جدایه برتر، بر میزان رشد طولی ساقه و ریشه گیاه عدس بررسی شد. نتایج: از 14 جدایه فیلوسفری و 16 جدایه ریزوسفری، 2 جدایه الف و ب که به ترتیب از ریزوسفر و فیلوسفر گندم جداسازی شده بودند و بیشترین توانایی را در خصوص پارامتر‌های مورد نظر داشتند، براساس تعیین توالی بخشی از ژن 16S rDNA شناسایی شدند. جدایه الف با قابلیت تثبیت نیتروژن و محلول‌سازی فسفات به میزان 98 درصد مشابه باسیلوس سرئوس و جدایه ب با قابلیت تولید اکسین به میزان 99 درصد مشابه آرتروباکتر گلوبیفورمیس بود. جدایه A به میزان 8 درصد و جدایه B به میزان 50 درصد توانستند رشد طولی ساقه عدس را افزایش دهند. بحث و نتیجه‌گیری: ریزوسفر گندم دارای باکتری‌هایی با قابلیت تثبیت نیتروژن، محلول‌سازی فسفات و تولید اکسین است. به‌کارگیری چنین سویه‌هایی، می‌تواند نقش موثری در بهبود رشد گیاهان و کاهش مصرف کودهای شیمیایی داشته باشد. | ||
| کلیدواژه‌ها | ||
| ایندول استیک اسید؛ تثبیت نیتروژن؛ محلول‌سازی فسفات | ||
| عنوان مقاله [English] | ||
| Isolation and Molecular Identification of Plant Growth-Promoting Bacteria from Phyllosphere and Rhizosphere of Triticum aestivum L. | ||
| نویسندگان [English] | ||
| Farahnaz Javanmardi1؛ Seyed Mohammad Mehdi Mahmoodi2؛ Hasan Rostami3 | ||
| 1Assistant Professor, Department of Biology, Faculty of Basic Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran | ||
| 2Assistant Professor, Department of Biology, Faculty of Basic Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran | ||
| 3MSC.Department of Microbiology, Faculty of Basic Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran | ||
| چکیده [English] | ||
| Introduction: A significant number of soil bacteria are capable of nitrogen fixation, phosphate solubilization and auxin production. Such bacteria can play an effective role in improving plant growth. The purpose of this research was to isolate, identify and investigate the performance of such bacteria on the growth rate of lentil. Materials and methods: Thirty bacterial samples were isolated from phyllosphere and rhizosphere of wheat. Nitrogen-free culture medium was used to identify nitrogen-fixing bacteria, and NBRIP culture medium was used to evaluate the ability of solubilization of phosphate. The ability of the isolates to produce auxin was investigated by the Salkowski method. Also, the effect of two superior isolates on the longitudinal growth of stem and root of lentil were investigated. Results: Out of 14 phyllospheric and 16 rhizospheric isolates, 2 isolates A and B, which were isolated from the rhizosphere and phyllosphere of wheat, respectively, and had the highest ability for the desired parameters, were identified based on sequencing a portion of 16S rDNA gene. Isolate A with nitrogen fixation and phosphate solubilization ability was 98% similar to Bacillus cereus and isolate B with auxin production ability was 99% similar to Arthrobacter globiformis. isolate A by 8% and isolate B by 50% were able to increase the longitudinal growth of lentil stem. Discussion and Conclusion: The wheat rhizosphere have bacteria capable of nitrogen fixation, phosphate solubilization, and auxin production. Applying such strains can play an effective role in improving plant growth and reducing the use of chemical fertilizers. | ||
| کلیدواژه‌ها [English] | ||
| : Indole acetic acid, Nitrogen fixation, Phosphate solubilization | ||
| مراجع | ||
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