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بررسی ریزریختشناسی برگ برخی از گونه‌های بنفشه (Viola spp.) در ایران (ارسال مجدد) | ||
| زیست شناسی کاربردی | ||
| مقاله 6، دوره 37، شماره 3 - شماره پیاپی 81، آذر 1403، صفحه 61-70 اصل مقاله (884.05 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2024.48408.1650 | ||
| نویسندگان | ||
| ماهیده حبیبی کلیبر1؛ سارا خراسانی نژاد* 2؛ مصطفی عبادی نهاری3؛ قاسم کریم زاده4؛ محسن فرهادپور5؛ کریستین گوربر6 | ||
| 1دانشجوی دکتری، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، دانشکده تولید گیاهی، گروه علوم باغبانی، گرگان، ایران | ||
| 2دانشیار (فیزیولوژی و اصلاح گیاهان دارویی) گروه علوم باغبانی، دانشکده تولیدات گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان | ||
| 3دانشیار، دانشگاه شهید مدنی آذربایجان، دانشکده علوم پایه، گروه زیست‌شناسی، تبریز، ایران | ||
| 4استاد، دانشگاه تربیت مدرس تهران، پردیس کشاورزی، گروه ژنتیک و به‌نژادی گیاهی، تهران، ایران | ||
| 5استادیار، پژوهشگاه ملی ژنتیک و زیست فناوری، پژوهشکده زیست فناوری کشاورزی، گروه زیست‌فرآورده‌های گیاهی، تهران، ایران | ||
| 6دانشیار، دانشگاه پزشکی وین، مرکز فیزیولوژی و فارماکولوژی، وین، اتریش | ||
| چکیده | ||
| مقدمه: سرده بنفشگان (Viola L.) بزرگترین سرده از تیره بنفشه بوده و شامل 600-525 گونه است که 19 گونه از آن بومی ایران است. روش‌ها: تحقیق حاضر برای نخستین بار اقدام به بررسی ریزریختشناسی صفات برگ از قبیل شکل، ابعاد و تراکم روزنه و کرک در 6 گونه بنفشه با استفاده از میکروسکوپ الکترونی نگاره (SEM) با هدف شناسایی صفات مفید جهت تفکیک و تشخیص گونه‌ها نمود. خصوصیات برگ از قبیل ابعاد و تراکم روزنه و کرک به وسیله نرم‌افزار Image J v 1.52 اندازه‌گیری شد. آنالیز داده‌ها از صفات ریزریختشناسی برگ با نرم‌افزارSPSS v 26 انجام شد. برای تعیین میزان قرابت گونه‌ها از روش تحلیل خوشه‌ای WARD استفاده گردید. نتایج و بحث: نتایج آنالیز میکروسکوپی گونه‌ها را از لحاظ ویژگی‌های ریز‌ریختی، به 7 نوع تیپ کرکی و روزنه‌ها را به 4 گروه تفکیک کرد. نتایج آنالیز میکروسکوپی نشان داد که کرک‌ها در گونه‌های مورد مطالعه به شکل مخروطی، مثلثی‌شکل و بندبند و ۳ حالت خوابیده، افراشته و نیمه‌افراشته وجود دارند بلندترین کرک در گونه V. hirta مشاهده شد. روزنه‌ها در همه گونه‌ها از لحاظ شکل به‌صورت تخم‌مرغی شکل دیده شدند. بزرگترین ابعاد روزنه مربوط به گونه V. occulta و بیشترین تراکم روزنه هم مربوط به گونه V. hirtaبود. نتایج نشان داد که صفات ریزریختشناسی برگ در ۶ گونه بنفشه به‌ویژه گونه V. hirta می‌تواند در شناسایی و تفکیک گونه‌ها مفید باشد. | ||
| کلیدواژه‌ها | ||
| : بنفشه؛ تاکسونومی؛ روزنه؛ کرک | ||
| عنوان مقاله [English] | ||
| Leaf micromorphology of some Viola spp. from Iran (resent) | ||
| نویسندگان [English] | ||
| Mahideh Habibi kaleibar1؛ sarah khorasaninejad2؛ Mostafa Ebadi Nahari3؛ Ghasem Karimzadeh4؛ mohsen farhadpour5؛ Christian Gruber6 | ||
| 1PhD student of medicinal plant, Horticultural Sciences Department, Plant Production Faculty, University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| 2Associate Professor (Physiology and breeding of Medicinal Plants) Department of Horticultural Sciences, Plant Production Faculty, Gorgan University of Agricultural Sciences and Natural Resources | ||
| 3Associate Professor, Shahid Madani University of Azerbaijan, Faculty of Basic Sciences, Department of Biology, Tabriz, Iran | ||
| 4Professor, Department of Biotechnology and Plant Breeding, Tarbiat Modares University, Tehran, Iran | ||
| 5Assistant Professor, National Genetics and Biotechnology Research Institute, Agricultural Biotechnology Research Institute, Department of Plant Bioproducts, Tehran, Iran | ||
| 6Associate Professor, Medical University of Vienna, Center for Physiology and Pharmacology, Vienna, Austria | ||
| چکیده [English] | ||
| Introduction: The genus Viola is the largest genus of the Violaceae and includes 525-600 species, of which 19 species are native to Iran. Methods: For the first time, micromorphological studies of leaf characters such as shape, dimensions, and density of stomata and trichomes in 6 species of Violets were carried out using scanning electron microscopy (SEM) to identify useful characters for the identification of species. The characteristics of the leaf such as the dimensions and density of stomata and trichome were measured by image J V.1.52 software. The information was obtained from leaf micromorphological properties were analyzed using SPSS V. 26. Cluster analysis was used by WARD method to determine the species affinity. Results and discussion: The results of the microscopic analysis of the species in terms of micromorphological properties, classified 7 types of trichomes and stomata into 4 groups. The results of the microscopic analysis showed that the trichomes in the studied species are conical, triangular, and uncinate in shape, and there are 3 states: lying, raised, and semi-raised. The longest trichome in V. hirta observed. In all species, stomata were seen as oval in shape. The largest stomatal size was related to V. occulta species and the highest stomatal density was also related to V. hirta species. The results showed that micromorphological characters of six species of viola species, especially V. hirta species identification and separation. | ||
| کلیدواژه‌ها [English] | ||
| Stomata.Taxonomy, Trichome, Violet | ||
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