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خصوصیات فیزیکوشیمیایی و ایمونولوژیکی RiVax بارگذاری شده در نانوذرات PLGA | ||
زیست شناسی کاربردی | ||
مقاله 7، دوره 37، شماره 3 - شماره پیاپی 81، آذر 1403، صفحه 71-82 اصل مقاله (961.94 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22051/jab.2024.46557.1616 | ||
نویسندگان | ||
داود صادقی1؛ مصطفی بخشی2؛ میر مرتضی سادات ابراهیمی3؛ شهرام نظریان4؛ مهدی زین الدینی* 5 | ||
1استادیار ،مرکززیست شناسی، دانشکده علوم پایه، دانشگاه جامع امام حسین علیه السلام | ||
2دانشآموخته دکترا مرکز علم و فناوری زیست شناسی، دانشکده علوم پایه، دانشگاه جامع امام حسین(ع) | ||
3دانشجوی دکترا،مرکززیست شناسی، دانشکده علوم پایه، دانشگاه جامع امام حسین علیه السلام | ||
4دانشیار ،گروه زیست شناسی دانشکده علوم پایه دانشگاه جامع امام حسین(ع) | ||
5دانشیار،مرکز علوم زیستی و زیست فناوری، دانشگاه صنعتی مالک اشتر، تهران، ایران | ||
چکیده | ||
مقدمه: ریسین یک سم گلیکوپروتئینی در گیاه کرچک می باشد که از دو زنجیره A (RTA) و B (RTB) تشکیل شده است. دو کاندید واکسن پروتئینی بر پایه زنجیره A شامل RiVax و RVEc بر مقابله با مسمومیت ناشی از این سم وجود دارد. اگرچه مطالعات مختلفی در مورد قدرت ایمنی زایی RiVax به تنهایی و با کمک ادجوانت انجام شده اما سیستمهای با قابلیت رهایش کنترل شده نوین مانند نانوذرات، جهت بهبود ایمنیزایی این پروتئین تا امروز مورد استفاده قرار نگرفته است. هدف از این مطالعه ساخت نانوذرات پلیلاکتیک-کو-گلایکولیکاسید(PLGA) حاوی RiVax با سرعت رهایش آهسته و ارزیابی پارامترهای فیزیکوشیمیایی و پتانسیل ایمنیزایی این سیستم در مقایسه با پروتئین تنها میباشد. روشها: ابتدا پس از خالصسازی RiVax، از روش امولسیون آب-روغن-آب برای بارگذاری RiVax در نانوذرات PLGA استفاده شد. پس از بررسی ویژگیهای فیزیکوشیمیایی نانوذرات حاوی RiVax، آنها به گروههای مختلف موشی تجویز شدند. یافتهها: نتایج نشان دهنده این بود که با توجه به استفاده از پلیمر PLGA با نسبت پلی لاکتیک اسید بیشتر نسبت به پلی گلایکولیک اسید، الگوی آزادسازی پروتئین از نانوذره آهسته بود (12 درصد پروتئین در 40 روز) و نتایج ایمنسازی تفاوتی در پاسخ ایمنی نانوذرات حاوی RiVax نسبت به RiVax تنها نشان نداد. نتیجهگیری: با وجود تحقیقات مختلف و نتایج مثبت در ارتباط با ماهیت ارتقاء ایمنیزایی توسط نانوذرات PLGA یکی از عوامل مهم در ارتقاء ایمنیزایی این نانوذرات نسبت اسید لاکتیک به اسید گلیکولیک تشکیل دهنده PLGA می باشد که تأثیر قابل توجهی بر میزان آزادسازی آنتی ژن و یا بر پاسخ ایمنی دارد. | ||
کلیدواژهها | ||
ایمنی زایی؛ پروتئین RiVax؛ ریسین؛ نانوذرات PLGA | ||
عنوان مقاله [English] | ||
Physicochemical and Immunological Properties of RiVax Loaded in PLGA nanoparticles | ||
نویسندگان [English] | ||
Davoud Sadeghi1؛ Mostafa Bakhshi2؛ Mir-Morteza Sadat Ebrahimi3؛ Shahram Nazarian4؛ Mehdi Zeinoddini5 | ||
1Assistant Professor.Department of Biology, Faculty of Basic Sciences, Imam Hossein University, | ||
2PhD student.Biology Dept. Faculty of Basic Sciences, Imam Hossein Uni. | ||
3PhD student.Biology Dept., Faculty of Basic Sciences, Imam Hossein Uni. | ||
4Associate Professor.Biology Dept. Faculty of Basic Sciences, Imam Hosein Uni. | ||
5Associate Professor.Center for Biological Sciences and Biotechnology, Malik Ashtar University of Technology | ||
چکیده [English] | ||
Introduction: Ricin is a glycoprotein poison found in the castor plant, which consists of two chains A (RTA) and B (RTB). There are two protein vaccine candidates based on chain A including RiVax and RVEc, against poisoning caused by this toxin. Although various studies have been conducted on the immunogenicity of RiVax alone and with the help of adjuvant, modern controlled release systems such as nanoparticles have not been used to improve the immunogenicity of this protein. The aim of this study is to manufacture polylactic-co-glycolic acid (PLGA) nanoparticles containing RiVax with a slow release rate and to evaluate the physicochemical parameters and immunogenic potential of system compared to protein alone. Methods: First, after purifying RiVax, the water-oil-water emulsion method was used to load RiVax into poly lactic acid-co-glycolic acid (PLGA) nanoparticles. After investigating the physicochemical properties of NPs containing RiVax, they were administered to different groups of mice. Results: The results showed that due to the use of PLGA polymer with a higher ratio of polylactic acid than polyglycolic acid, the protein release pattern from the nanoparticle was slow (12% protein in 40 days) and the immunization results showed a difference in response. Safety of nanoparticles containing RiVax compared to RiVax alone was not demonstrated. Conclusion: it was determined that despite the various researches and positive results related to the nature of immunogenicity enhancement by PLGA nanoparticles, one of the important factors in the enhancement of immunogenicity of these nanoparticles is the ratio of lactic acid to glycolic acid | ||
کلیدواژهها [English] | ||
Immunization, PLGA nanoparticles (NPs), Ricin, RiVax protein | ||
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مراجع | ||
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