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مطالعه پاسخ‌های مورفوفیزیولوژیک و بیوشیمیایی گیاه گوجه فرنگی تیمار شده با سوربات پتاسیم تحت تنش Alternaria solani | ||
| زیست شناسی کاربردی | ||
| مقاله 7، دوره 37، شماره 2 - شماره پیاپی 80، شهریور 1403، صفحه 73-85 اصل مقاله (922.46 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2024.46284.1610 | ||
| نویسندگان | ||
| لیلی هنرمند1؛ نادعلی باباییان جلودار* 2؛ نادعلی باقری3؛ علی دهستانی4؛ ولی اله بابائی زاد5 | ||
| 1دانشجوی دکترا ،دانشگاه علوم کشاورزی و منابع طبیعی ساری | ||
| 2استاد،گروه اصلاح نباتات و بیوتکنولوژی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران | ||
| 3دانشیار ،گروه اصلاح نباتات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران. | ||
| 4دانشیار ،موسسه ژنتیک و بیوتکنولوژی کشاورزی طبرستان (GABIT)، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران. | ||
| 5دانشیار ،گروه گیاهپزشکی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران. | ||
| چکیده | ||
| مقدمه: بیماری لکه موجی از بیماری‌های مهم گوجه‌فرنگی بوده و کاربرد قارچ‌کش‌ها رایج‌ترین روش مدیریت آن می‌باشد. به‌‌دلیل مضرات زیست‌محیطی قارچ‌کش‌ها، استفاده از ترکیبات جایگزین کم‌خطر و القاگر‌های دفاعی مورد‌توجه قرار گرفته است. القاگر‌های دفاعی، موجب تغییرات فیزیولوژیک تخصصی در گیاه شده و سیستم‌های دفاعی میزبان را در برابر تنش تقویت می‌کنند. در این مطالعه، تأثیر القاگر سوربات‌پتاسیم (KS) روی پاسخ‌های فیزیولوژیک، بیوشیمیایی و دفاعی گوجه‌فرنگی تحت تنش قارچ بیماری‌زا Alternaria solani ارزیابی شد. روش‌ها: این تحقیق به صورت اسپلیت‌پلات -فاکتوریل با سه فاکتور، حضور یا عدم حضور قارچ بیماری‌زا، استفاده از KS یا آب مقطر (شاهد) برای محلول‌پاشی و سه دوره‌‌زمانی برای نمونه‌برداری (24، 72 و 168 ساعت پس از تلقیح) اجرا گردید. نتایج و بحث: نتایج نشان داد که وزن تر و خشک در گیاهان تلقیح‌شده، از گیاهان شاهد کمتر بود. بیماری‌زا سبب آسیب به غشای سلولی گیاه و رنگیزه‌های فتوسنتزی شد و مقدار MDA و Chl-a در گیاهان تلقیح‌شده نسبت به گیاهان تلقیح‌نشده به‌طور معنی‌داری کاهش یافت. میزان فعالیت آنزیم‌های POD و CAT در گیاهان تیمار‌شده با KS نسبت به شاهد به‌طور معنی‌داری افزایش نشان داد. میزان فعالیت کیتیناز در گیاهان تیمار‌شده با KS، در 168 ساعت پس از تلقیح افزایش بسیار معنی‌داری نشان داد که می‌تواند نشانه‌ای از تحریک سیستم ایمنی باشد. نتایج نشان داد که القاگر KS توانست روی وزن و رشد گیاهان مؤثر باشد و با افزایش فعالیت آنزیم‌های آنتی‌اکسیدانی، اثرات منفی تنش زیستی در گیاهان را بهبود بخشد. | ||
| کلیدواژه‌ها | ||
| آنزیم‌های دفاعی؛ بلایت زودرس؛ قارچ بیماری زا؛ مقاومت القایی | ||
| عنوان مقاله [English] | ||
| Studying the morphophysiological and biochemical responses of tomato treated with potassium sorbate under Alternaria solani stress | ||
| نویسندگان [English] | ||
| Leyli Honarmand1؛ Nadali Babaeian Jelodar2؛ Nadali Bagheri3؛ Ali Dehestani4؛ Valiollah Babaezad5 | ||
| 1MSC.Agricultural Sciences and Natural Resources University | ||
| 2Porfosor.Department of Plant Breeding, Sari Agricultural Sciences and Natural Resources University | ||
| 3Associate Professor.Plant Breeding Department, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. | ||
| 4Associate Professor.Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, Sari, Iran. | ||
| 5Associate Professor.Department of Plant Protection, Sari Agricultural Sciences and Natural Resources University, Sari, Iran | ||
| چکیده [English] | ||
| Introduction: Early blight is one of the most significant tomato diseases and fungicide application is the most common measure used to control its damage. Exploitation of generally regarded as safe (GRAS) and defense inducers have been focused on as alternative strategies to avoid environmental impacts of fungicides. Defense inducers enhance plant defense system against stresses through inducing specific changes in plant physiological characteristics. In the present study the effect of potassium sorbate (KS) on physiological, biochemical and defense response of Alternaria solani-infected tomato plants was investigated. Methods This research was conducted as split plot-factorial with three factors, presence or absence of a pathogen, the use of either KS or distilled water (control) for foliar spraying, and three time courses for sampling (24, 72, and 168 hours). Results: The results showed that the fresh and dry weights in inoculated plants were lower than that of non-inoculated plants. The pathogen caused damage to the plant cell membrane and significantly reduced photosynthetic pigments. Additionally, the amount of MDA and chla in inoculated plants was significantly decreased compared to non-inoculated plants. The activity of POD and CAT enzymes significantly increased in plants treated with KS compared to the control. Chitinase enzyme in plants treated with KS showed a significant increase in the inoculated plants 168 hours, which can be a sign of immune system stimulation. The results revealed that the KS inducer was effective on the weight and growth of plants and reduced biotic stress damages through increased activity of antioxidant and defense enzyme | ||
| کلیدواژه‌ها [English] | ||
| Defense enzymes, Early blight, Induced resistance, Pathogenic fungus | ||
| مراجع | ||
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آمار تعداد مشاهده مقاله: 54 تعداد دریافت فایل اصل مقاله: 50 |
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