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کلونیزاسیون اندوفیتی ریشه برنج با آزوسپریلوم ایراکنز Azosprillum irakense و اثر آن بر برخی شاخص‌های رشد و بیوشیمیایی | ||
| زیست شناسی کاربردی | ||
| مقاله 7، دوره 37، شماره 1 - شماره پیاپی 79، خرداد 1403، صفحه 87-101 اصل مقاله (966.63 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2023.42839.1542 | ||
| نویسندگان | ||
| سیده زینب شریف السادات1؛ مهناز اقدسی* 2؛ فائزه قناتی3؛ محمد حسین ارزانش4 | ||
| 1دانش آموخته دکتری، گروه زیست‌شناسی- فیزیولوژی گیاهی، دانشکده علوم، دانشگاه گلستان، گرگان، ایران | ||
| 2استاد، گروه زیست‌شناسی- فیزیولوژی گیاهی، دانشکده علوم، دانشگاه گلستان، گرگان، ایران | ||
| 3استاد، گروه بیولوژی گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران gangia@modares.ac.ir | ||
| 4استادیار، گروه تحقیقات خاک و آب، مرکز تحقیقات کشاورزی و منابع طبیعی گلستان، گرگان، ایران | ||
| چکیده | ||
| مقدمه: آزوسپریلوم یکی از انواع میکرورارگانیسم‌های تثبیت‌کننده نیتروژن مولکولی است که در همیاری با ریشه گیاهان تیره غلات و گرامینه‌های دیگر، رشد و نمو آن‌ها را تقویت می‌کند. هدف از تحقیق حاضر بررسی اثر باکتری آزوسپریلوم ایراکنز (Azosprillum irakense) به همراه تیمار هورمون‌های اکسین (IAA) و یا جیبرلین (GA3) بر برخی شاخص‌های رشد و بیوشیمیایی در گیاهچه‌های برنج رقم هاشمی (cv.hashemi Oryza sative) و مشاهده روند کلونیزاسیون باکتری در سلول‌های ریشه است. روش‌ها: به این منظور ریشه گیاهچه‌های 21 روزه برنج پس از تلقیح با آزوسپریلوم با غلظت‌های مختلف) 0, 100, 200 (ppm IAA و GA3 به تنهایی و یا در ترکیب با یکدیگر (ppm IAA 200 ppm GA3+200) به مدت 4 هفته تیمار شدند. نتایج و بحث: روند کلونیزاسیون باکتری شامل تشکیل زیست لایه، اضمحلال دیواره، ورود باکتری به داخل لایه‌ی پوست ریشه و تشکیل کلنی‌های منفرد و پراکنده است. وزن خشک و تر ریشه و اندام هوایی، وزن خشک و تر کل و سرعت رشد نسبی در نمونه‌های تلقیح‌شده که با هورمون‌ها نیز تیمار شده بودند، افزایش معنی‌داری یافت که این افزایش بخصوص در تیمار ترکیبی هورمون اکسین و جیبرلین قابل توجه بوده است. هم‌چنین نتایج نشان داد که محتوای پراکسید هیدروژن در ریشه، پروتئین و قند محلول و فسفر در اندام هوایی و ریشه گیاهچه‌های تیمارشده به طور معنی‌داری در مقایسه با شاهد افزایش یافته است. نتیجه‌گیری: باتوجه به نتایج حاضر، کاربرد باکتری آزوسپریلوم ایراکنز به همراه غلظت ترکیبی هورمون‌ها برای افزایش عملکرد و رشد گیاهچه‌های برنج موثر است. | ||
| کلیدواژه‌ها | ||
| :اضمحلال دیواره سلولی؛ آزوسپریلوم؛ اکسین؛ برنج؛ جیبرلین؛ شاخص‌های رشد | ||
| عنوان مقاله [English] | ||
| Endophytic colonization of rice root with Azospirillum irakense and its effect on some growth and biochemical parameters | ||
| نویسندگان [English] | ||
| Seyedeh Zeynab Sharifsadat1؛ Mahnaz Aghdasi2؛ Faezeh Ghanati3؛ Mohammad Hossein Arzanesh4 | ||
| 1PhD student, Department of Plant Biology-Physiology, Faculty of Science, Golestan University, Gorgan, Ira | ||
| 2Professor, Department of Biology-Plant Physiology, Faculty of Science, Golestan University, Gorgan, Iran | ||
| 3Professor, Department of Plant Biology, Faculty of Life Sciences, Tarbiat Modares University, Tehran, Iran | ||
| 4Assistant Professor, Soil and Water Research Department, Golestan Agriculture and Natural Resources Research Center, Gorgan, Iran | ||
| چکیده [English] | ||
| Introduction: Azospirillum is the nitrogen fixing microorganisms which improves the growth and development of cereals and other gramineae plants in association with their roots. The goal of present study was to investigate the effect of Azospirillum irakense inoculation in combinations with or without auxin (IAA) and/or gibberellin (GA3) treatment on the growth and biochemical parameters of rice (Oryza sative cv. hashemi) seedlings, as well as observing the bacterial colonization process in the root cells. Methods: The roots of 21-days-old rice seedlings were treated with different concentrations (0, 100, 200 ppm) of IAA and/or GA3 for 4 weeks after inoculation with Azosprillum. Result & discussion: The process of bacterial colonization involves the formation of a biofilm, disintegration of the wall, entry of bacteria into root parenchymal cells and development of isolated and scattered colonies. Dry and fresh weight of roots and aerial parts, total dry and fresh weight, and relative growth rate was significantly increased after inoculation with bacteria. The highest level of these parameters was obtained in the seedlings which inoculated with bacteria in combinations with IAA plus GA3 treatment. Meanwhile, the amount of hydrogen peroxide in the roots, soluble protein, soluble sugar and phosphorus was significantly increased in both roots and aerial parts of seedlings after inoculation with bacteria in combinations with IAA plus GA3 treatment, compared to the control. Conclusion: According to the current results, the application of Azospirillum irakense in combinations with IAA and GA3 treatment is a powerful strategy to improve rice seedlings growth | ||
| کلیدواژه‌ها [English] | ||
| Azospirillum, Auxin, Destruction of cell wall, Gibberellin, Growth indicators, Rice | ||
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