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تأثیر روش نگهداری محلول نایل رد بر شدت فلوروسانس در تشخیص لیپیدها | ||
| زیست شناسی کاربردی | ||
| مقاله 8، دوره 36، شماره 2 - شماره پیاپی 76، شهریور 1402، صفحه 126-140 اصل مقاله (1.52 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2023.41939.1516 | ||
| نویسندگان | ||
| زهره نوروزی مطلق1؛ محمود اخوان مهدوی* 2؛ رضا قشلاقی2 | ||
| 1دانشجوی دکتری، گروه مهندسی شیمی، دانشکده مهندسی، دانشگاه فردوسی مشهد، مشهد، ایران | ||
| 2دانشیار، گروه مهندسی شیمی، دانشکده مهندسی، دانشگاه فردوسی مشهد، مشهد، ایران. | ||
| چکیده | ||
| نایل رد یک رنگ فلورسنت است که برای تعیین میزان لیپید درون سلولی استفاده میشود. این روش میتواند با دقت بالا مقادیر بسیار کم لیپید درون سلولی را شناسایی کند. اما شدت فلورسانس نایل رد با زمان تغییر میکند و همین مساله استفاده از آن را برای مجموعه آنالیزهایی که در یک فاصله زمانی انجام میشوند با مشکل مواجه میکند. با توجه به اینکه استفاده از محلول نایل رد تازه برای تمام آنالیزها امکان پذیر نیست. پس بایدروشهایی برای حفظ شدت فلورسانس محلول نایل رد باید بکار گرفته شود تا بتوان از این معرف با اطمینان برای آنالیز لیپید استفاده نمود. در این مطالعه شدت فلورسانس محلول نایل رد با توجه به دو عامل زمان و تعداد دفعات ذوب و انجماد بررسی شده است. نتایج نشان داد که شدت فلورسانس محلول نایل رد در اثر انجماد کاهش مییابد. اما اگر محلول منجمد شده تا زمان استفاده منجمد باقی بماند و فقط در هنگام مصرف ذوب شود، میتوان آن را برای مدت طولانی نگهداری کرد و کاهش شدت فلورسانس کمتر از 10درصد خواهد بود. اما اگر به دفعات ذوب و منجمد شود (4 بار یا بیشتر) شدت فلورسانس تا حدود 80 درصد کاهش مییابد که دیگر برای آنالیز مناسب نیست. در صورتی که از معرف منجمد شده (یکبار منجمد شده) استفاده شود برای محدوده غلظت لیپید (روغن زیتون) صفر تا 4 میکروگرم بر میلی لیتر غلظت 0.05میکروگرم بر میلی لیتر از نایل رد مناسب است و لیپید را بدرستی شناسایی و غلظت آن را تعیین میکند. | ||
| کلیدواژهها | ||
| رنگ نایل رد؛ روغن زیتون؛ فلورسنت | ||
| عنوان مقاله [English] | ||
| The effect of the storage method of Nile red solution on the fluorescence intensity in lipids detection | ||
| نویسندگان [English] | ||
| Zohreh Noruzi Motlagh1؛ Mahmood Akhavan Mahdavi2؛ Reza Gheshlaghi2 | ||
| 1PhD student, Department of Chemical Engineering, Faculty of Engineering, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 2Associate Professor, Department of Chemical Engineering, Faculty of Engineering, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| چکیده [English] | ||
| Nile red is a fluorescent dye that is used to determine the amount of intracellular lipids. This method can detect very low amounts of intracellular lipid with high accuracy. But the intensity of Nile red fluorescence changes with time, and this problem makes it difficult to use it for a set of analyzes that are performed in a time interval. Considering that it is not possible to use fresh Nile red solution for all analyses. So, methods should be used to maintain the fluorescence intensity of Nile red solution so that this reagent can be used reliably for lipid analysis. In this study, the fluorescence intensity of Nile red solution has been investigated according to the two factors of time and the number of times of melting and freezing. The results showed that the fluorescence intensity of Nile red solution decreases due to freezing. But if the frozen solution remains frozen until use and is only melted during use, it can be stored for a long time and the decrease in fluorescence intensity will be less than 10%. But if it is repeatedly thawed and frozen (4 times or more), the fluorescence intensity decreases to about 80%, which is no longer suitable for analysis. If the frozen reagent (once frozen) is used, for 0 to 4 μg/ml of lipid concentration (olive oil) from , 0.05 μg/ml of Nile red concentration is suitable, and the lipid is correctly identified and its concentration is determined. | ||
| کلیدواژهها [English] | ||
| Fluorescent, Nile red dye, olive oil | ||
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