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بررسی اثر کلر بر زنده مانی و تغییرات فراساختاری Acanthamoeba castellanii | ||
| زیست شناسی کاربردی | ||
| مقاله 3، دوره 36، شماره 1 - شماره پیاپی 75، خرداد 1402، صفحه 67-84 اصل مقاله (1.47 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2022.41608.1510 | ||
| نویسندگان | ||
| زینب اسدی1؛ سمیه بهرامی* 2؛ مهدی زارعی3؛ حسین حمیدی نجات4 | ||
| 1دانشجوی دکترا، بخش انگل‌شناسی، دانشکده دامپزشکی دانشگاه شهید چمران اهواز، اهواز، ایران | ||
| 2دانشیار، بخش انگل شناسی دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران | ||
| 3استاد، بخش بهداشت مواد غذایی دانشگاه شهید چمران اهواز، اهواز، ایران | ||
| 4استاد، بخش انگل شناسی دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران | ||
| چکیده | ||
| مقدوه: از کلر به عنوان یک گندزدای موثر در از بین بردن میکروارگانیسم‌های بیماری‌زا در سیستم تصفیه آب شهری استفاده می‌شود. با این حال مطالعات نشان داده‌اند که Acanthamoeba از منابع آبی مختلف کشورمان علی‌رغم تصفیه جدا می‌شود. در این مطالعه به بررسی اثر غلظت‌های متداول کلر که در سیستم تصفیه آب شهری استفاده می‌شود بر زنده‌مانی Acanthamoeba castellanii و تغییرات فراساختاری ناشی از آن پرداخته شده‌است. روش‌ها: تروفوزوئیت‌ها و کیستهایAcanthamoeba در زمان‌های مختلف (30 دقیقه، 1 و 2 ساعت) با غلظت‌های مختلف (ppm10-1) هیپوکلریت کلسیم، مواجه شدند. برای بررسی تغییرات فراساختاری تروفوزوئیت آمیب از میکروسکوپ الکترونی نگاره و گذاره استفاده شد. نتایج و بحث: نتایج این مطالعه نشان داد که غلظت‌های متداول کلر قادر به از بین بردن کامل تروفوزوئیت‌ها و کیستهای A. castellanii نمی‌باشند. همچنین کیست‌ها به غلظت‌های مختلف کلر مقاوم‌تر بوده و در زمان و غلظت برابر کلر، درصد کمتری از کیست‌ها در مواجه با کلر از بین می‌روند. تغییر نفوذپذیری غشای سلولی، کاهش تعداد پاهای کاذب، افزایش میتوکندری، واکوئله شدن سیتوپلاسم و تغییر در شبکه‌ی اندوپلاسمی از تغییرات فراساختاری ایجاد شده در آمیب بود. این مطالعه ضرورت بازنگری دستورالعمل بکارگیری گندزداهایی نظیر کلر را در تصفیه‌ی سیستم آب شهری مطرح می-کند. چرا که علاوه بر بیماری‌زا بودن آمیب، مخزن بودن آن و انتقال عوامل میکروبی حائز اهمیت است. | ||
| کلیدواژه‌ها | ||
| آکانتاموبا کستلانی؛ تغییرات فراساختاری؛ کلر؛ زنده‌مانی | ||
| عنوان مقاله [English] | ||
| Effect of chlorine on survival and ultrastructural changes of Acanthamoeba castellanii | ||
| نویسندگان [English] | ||
| Zeinab Asadi1؛ Somayeh Bahrami2؛ Mehdi Zarei3؛ Hossein Hamidinejat4 | ||
| 1Ph. D student, Department of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 2Associate Professor, Department of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 3Professor, Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 4Professor, Department of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| چکیده [English] | ||
| Abstract Introduction: Chlorine is an effective disinfection agent to kill pathogenic microorganisms in the municipal water treatment system. However, despite treatment, studies have shown that Acanthamoeba is isolated from different water sources in Iran. In this study, the effect of standard concentrations of chlorine used in urban water treatment systems was evaluated on the survival of Acanthamoeba castellanii and its ultrastructure. Materials and methods: Acanthamoeba trophozoites and cysts were exposed to different concentrations (1-10 ppm) of calcium hypochlorite at different times (30 minutes, 1 and 2 hours). Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) were used to study the ultrastructural changes of amoebic trophozoite. Results: This study showed that conventional chlorine concentrations could not completely eliminate A. castellanii trophozoites and cysts. Cysts were more resistant to different chlorine concentrations and compared to trophozoites, fewer cysts were killed at the same chlorine concentration and exposure time. Alteration of the cell membrane permeability, decrease in the number of pseudopodia, increase in mitochondria, vacuolation of the cytoplasm, and changes in the endoplasmic reticulum were the main ultrastructural changes in the chlorine-treated amoeba. Conclusion: This study showed that standard chlorine concentrations used as a disinfectant could not eliminate the trophozoites and cysts of A. castellanii. Due to the pathogenicity of the amoeba and its role as the reservoir and transmission of microbial agents, revising the guidelines for using disinfectants such as chlorine in the treatment of urban water systems is highlighted by this study. | ||
| کلیدواژه‌ها [English] | ||
| Acanthamoeba castellanii, Chlorine, Survival, Ultrastructural changes | ||
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| مراجع | ||
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