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بررسی اثر پیشتیمار اپیبراسینولید و اسپرمین بر برخی پارامترهای رشدی، فیزیولوژیکی و بیان ژنSOS1 وNHX1 در گیاه کدو (Cucurbita pepo L.) تحت تنش شوری | ||
زیست شناسی کاربردی | ||
دوره 35، شماره 4 - شماره پیاپی 74، اسفند 1401، صفحه 99-118 اصل مقاله (1.39 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22051/jab.2022.39878.1478 | ||
نویسندگان | ||
فاطمه نژادعلیمرادی* 1؛ فاطمه نصیبی2؛ محبوبه شیخ بهایی3 | ||
1استادیار گروه زیست شناسی، دانشگاه پیام نور، تهران، ایران | ||
2دانشیار گروه زیست شناسی، دانشگاه شهید باهنر کرمان، کرمان، ایران | ||
3دانشجوی دکترای ژنتیک، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران | ||
چکیده | ||
مقدمه: پتانسیل مثبت براسینواستروئیدها و پلی آمین ها به عنوان عوامل محافظ برای تنشهای محیطی ثابت شده است. روشها: در این پژوهش، گیاه Cucurbita pepoبا 24-اپیبراسینولید (EBL)(0، 01/0 و 1/0 میکرومولار) و اسپرمین (Spm) (0 و 1/0 و 1 میلیمولار) پیشتیمار شد و سپس تیمار شوری (0، 40 و 80 میلیمولار سدیم کلرید)، اعمال شد. نتایج و بحث: تنش شوری، وزن خشک، مقدار پتاسیم و کلسیم را کاهش و محتوای سدیم را افزایش داد. EBL و Spmمنجر به افزایش پتاسیم، کلسیم و کاهش سدیم اندام هوایی شد. تنش شوری بیان ژن SOS1 ریشه را افزایش داد و اثری بر بیان ژن NHX1 نداشت. EBL و Spm بیان ژن SOS1 را کاهش داد در حالیکه Spm بیان ژن NHX1 را افزایش داد؛ بنابراین بهنظر میرسد Spm از طریق بیشبیان ژن NHX1 و کدهبندی یون Na+ اضافی به درون واکوئل و حفظ محیط مطلوبتر منجر به افزایش مقاومت به تنش شوری و بهبود رشد گیاه میشود. | ||
کلیدواژهها | ||
پلیآمین؛ تنظیم کننده رشد گیاهی؛ تنش اسمزی؛ ژن ناقل سدیم | ||
عنوان مقاله [English] | ||
The effect of pretreatment of Epibracinolide and Spermine on some growth, physiological parameters and SOS1 and NHX1 gene expression in Pumpkin (Cucurbita pepo L.) under salinity stress | ||
نویسندگان [English] | ||
Fatemeh Nejad-Alimoradi1؛ Fatemeh Nasibi2؛ Mahboobeh Sheikhbahaei3 | ||
1Assistant Professor, Department of Biology, Payam Noor University, Tehran, Iran | ||
2Associate Professor, Department of Biology, Shahid Bahonar University of Kerman, Kerman, Iran | ||
3PhD student in Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran | ||
چکیده [English] | ||
Introduction: The positive potential of brassinosteroids and polyamines as protective agents for environmental stresses has been proven. Methods: In this study, Cucurbita pepo was pretreated with 24-epibrasinolide (EBL) (0, 0.01 and 0.1 μM) and spermine (0, 0.1 and 1 mM) and then salinity treatment (0, 40 and 80 mM sodium chloride) was applied. Results and discussion: Salinity stress decreased dry weight, potassium and calcium content and increased sodium content. EBL and Spm increased potassium, calcium and decreased sodium in shoots. Salinity stress increased root SOS1 gene expression and had no effect on NHX1 gene expression. EBL and Spm decreased expression of SOS1 gene while Spm increased expression of NHX1 gene. Therefore, it seems that Spm via the over-expression of the NHX1 gene substantially increased the tolerance to stress by sequestering excess Na+ into the vacuoles and sustaining a better cellular environment for improvement of plant growth under salinity stress. | ||
کلیدواژهها [English] | ||
: Polyamine, Plant growth regulator, Osmotic stress, Sodium transporter gene | ||
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مراجع | ||
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