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جداسازی و شناسایی باکتری‌های اندوفیت ریشه گلرنگ (Carthamus tinctorius) و بررسی تأثیر آنها بر جوانه‌زنی بذر و رشد گیاه | ||
| زیست شناسی کاربردی | ||
| دوره 35، شماره 3 - شماره پیاپی 73، آبان 1401، صفحه 143-160 اصل مقاله (723.77 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2022.37209.1445 | ||
| نویسندگان | ||
| شیدا نظرپور1؛ مریم محمدی سیچانی* 2؛ منیره رنجبر3 | ||
| 1دانشجوی کارشناسی ارشد ، گروه میکروبیولوژی، ,واحد فلاورجان، دانشگاه ازاد اسلامی، اصفهان، ایران | ||
| 2استادیار ، گروه میکروبیولوژی، واحد فلاورجان، دانشگاه آزاد اسلامی، اصفهان، ایران | ||
| 3استادیار گروه زیست شناسی، واحد فلاورجان، دانشگاه آزاد اسلامی | ||
| چکیده | ||
| امروزه باکتری های اندوفیت محرک رشد به عنوان کود زیستی بسیار مورد توجه قرار گرفته‌‌اند. هدف از این مطالعه شناسایی باکتری‌های اندوفیت ریشه گیاه گلرنگ و بررسی تأثیر آنها بر جوانه‌زنی بذر و رشد گیاه بود. سویه‌های اندوفیتی از ریشه گلرنگ جداسازی شدند و توانایی تولید اکسین و حل‌کنندگی فسفات آنها مورد ارزیابی قرار گرفت. اثر جدایه‌های منتخب در دو غلظت 105×5/1 و 108×5/1 باکتری در هر میلی‌لیتر بر روی جوانه‌زنی بذر گلرنگ و رشد آن مورد ارزیابی قرار گرفت. همچنین اثر ضدقارچی جدایه‌های اندوفیتی با روش پورپلیت ارزیابی شد. باکتری‌های اندوفیت Micrococcus luteus NMR ، Pseudomonas corrugota NMR ، Pseudomonas fluorescence NMR ، Pseudomonas brassicacearum NMR و Bacillus megaterium NMR از ریشه گلرنگ جدا شدند. در تیمار با سویه Bacillus megaterium NMR ، میانگین بذرهای جوانه‌زده هنگام تماس با غلظت cfu/ml 105×5/1، نسبت به هنگام تماس با غلظت cfu/ml 108×5/1، بهطور معناداری بیشتر بود. طول ساقه‌چه و ریشه‌چه در گروه شاهد نسبت به گروه تیمار به طور معناداری کمتر بود. ارتباط مستقیمی بین مقدار اکسین تولیدی جدایه‌های اندوفیتی و توانایی حلالیت فسفات آنها با افزایش طول ساقه‌چه، طول ریشه‌چه و تعداد بذرهای جوانه‌زده گلرنگ وجود داشت. ایزوله Pseudomonas brassicacearum NMR هر چهار نوع آنزیم پکتیناز، آمیلاز، پروتئاز و زایلاناز را تولید کرد. نتایج نشان داد ایزوله Pseudomonas fluorescence NMR رشد Aspergillus niger را به میزان 76/30 درصد مهار کرد. باکتری‌های اندوفیت جداسازی شده در تحقیق حاضر به دلیل قابلیت تولید اکسین و حلالیت فسفات و تأثیر مستتقیم آنها بر فاکتورهای رشد گلرنگ، به عنوان عوامل محرک رشد پیشنهاد می‌شوند. | ||
| کلیدواژه‌ها | ||
| باکتری های محرک رشد؛ ریشه گیاه؛ فعالیت ضدقارچی؛ گلرنگ | ||
| عنوان مقاله [English] | ||
| Isolation and identification of endophtic bacteria from safflower (Carthamus tinctorius) root and investigating their effect on seed germination and plant growth | ||
| نویسندگان [English] | ||
| Sheyda Nazarpour1؛ Maryam Mohammadi-Sichani2؛ Monireh Ranjbar3 | ||
| 1Msc, Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan,, Iran | ||
| 2Assistant Professor , Department of Microbiology, Falavarjan Branch, Islamic Azad university, Esfahan, Iran | ||
| 3Assistant Professor , Department of Biology, Falavarjan Branch, Islamic Azad university | ||
| چکیده [English] | ||
| The growth-promoting endophytic bacteria are highly regarded as bio-fertilizers. The aim of this study was to identify safflower endophytic bacteria and to detect their effects on seed germination and plant growth. Endophytic strains were isolated from safflower roots and their auxin production and phosphate solubility were evaluated. The effects of isolates were evaluated on the safflower seed germination and growth. Then, the activity of enzymes produced by the selected isolates were investigated. The antifungal effects of endophytic isolates were also evaluated by pour plate method. Endophytic bacteria including Micrococcus luteus, Pseudomonas corrugata, Pseudomonas fluorescens, Pseudomonas brassicacearum, and Bacillus megatrium were isolated from safflower roots. In treatment with Bacillus megatrium NMR isolate, the mean of germinated seeds by exposure to the concentration of 105 cfu/ml was significantly higher than concentration of 108 cfu/ml. Coleoptile length and root length in the control group were significantly shorter than the treatment groups. There was a direct relationship between the amount of auxin produced by endophytic isolates and their ability for phosphate solubility with increasing safflower coleoptile and root length, and germinated seeds. Pseudomonas brasicacearum NMR isolate produced all the four enzymes, pectinase, amylase, protease and xylanase. The Pseudomonas fluorescence NMR with the most effectiveness inhibited the growth of Aspergillus niger by 30.76%. The isolated endophytic bacteria in the present study are suggested as stimulants of plant growth in the field due to their ability to produce auxin and to dissolve phosphate and their direct effect on safflower growth factors. | ||
| کلیدواژه‌ها [English] | ||
| Antifungal effect, Growth-promoting bacteria, Plant root, Safflower | ||
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