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مطالعه عوامل مؤثر بر حذف زیستی اورانیوم توسط ریزجلبک زنده Chlorella vulgaris | ||
زیست شناسی کاربردی | ||
مقاله 7، دوره 34، شماره 4 - شماره پیاپی 70، بهمن 1400، صفحه 133-147 اصل مقاله (930.36 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22051/jab.2021.36140.1420 | ||
نویسندگان | ||
پریسا محمدی1؛ نورا جوانمردی2؛ پریسا تاجر محمد قزوینی* 3 | ||
1دانشیار ،گروه میکروبیولوژی، دانشکده علوم زیستی، دانشگاه الزهرا س، تهران، ایران | ||
2کارشناسی ارشد، گروه میکروبیولوژی، دانشکده علوم زیستی دانشگاه الزهرا (س)، تهران- ایران | ||
3استادیار ، پژوهشکده چرخه سوخت هستهای، پژوهشگاه علوم و فنون هستهای، تهران- ایران | ||
چکیده | ||
امروزه زیست پالایی به کمک ریزجلبک ها روشی موٴثر جهت حذف رادیونوکلییدها و فلزات سنگین از پساب ها مطرح هستند. زیست پالایی رادیونوکلییدها و فلزات سنگین روشی کارآمد در تصفیه پساب های آلوده به فلزات سنگین به شمار می رود. در این مطالعه زیست پالایی اورانیوم از محلولهای آبی با استفاده از ریز جلبک زنده Chlorella vulgaris در حالت ناپیوسته ارزیابی شد. روش پلاکت برمن توسط نرم افزار آماری Minitab جهت غربالگری عوامل موٴثر مانند غلظت اولیه اورانیوم، دما، زمان، pH و مقدار زیست توده بر حذف اورانیوم توسط ریزجلبکC. vulgaris استفاده شد. نتایج نشان دادند که عوامل غلظت اولیه اورانیوم و pH از نظر آماری توسط نرم افزار به عنوان عوامل موٴثر می باشند. بهینه سازی عوامل موٴثر در زیست پالایی اورانیوم توسط روش سطح پاسخ (RSM) مورد ارزیابی قرار گرفت. جهت تعیین اثرات اصلی و برهمکنش عوامل موٴثر بر حذف اورانیوم توسط C. vulgaris از طراحی مرکب مرکزی (CCD) استفاده شد. پس از آن پردازش دادههای تجربی و ارزیابی تطابق معادله با دادههای تجربی انجام شد و سپس مقادیر شرایط بهینه حذف تعیین شد. در نهایت نتایج نشان دادند که C. vulgaris در شرایط بهینه پیشنهاد شده توسط نرم افزارDesign-Expert قادر به حذف 63/99 درصد از اورانیوم موجود از محلولهای آبی حاوی 1/7 میلی گرم بر لیتر اورانیوم در 3/4 pH می باشد. | ||
کلیدواژهها | ||
ریزجلبک؛ زیست پالایی؛ طراحی آزمایش؛ اورانیوم؛ فلزات سنگین | ||
عنوان مقاله [English] | ||
Study of effective factors on biological removal of uranium by live microalgae Chlorella vulgaris | ||
نویسندگان [English] | ||
Parisa Mohammadi1؛ Nora javanmardy2؛ Parisa Tajer Mohammad Ghazvini3 | ||
1Associate Professor, Department of Microbiology, Faculty of Life Sciences, Al-Zahra University, Tehran, Iran | ||
2Msc., Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran | ||
3Assistant Professor, Nuclear Fuel Cycle Research Institute, Nuclear Science and Technology Research Institute, Tehran-Iran | ||
چکیده [English] | ||
Today bioremediation by microalga is an effective method to remove radionuclides and heavy metals from wastewater. Bioremediation of radionuclides and heavy metals is an efficient method of treating heavy metal contaminated effluents. In this study, the bioremediation of uranium from aqueous solutions was evaluated using live microalgae Chlorella vulgaris in a batch system. The Plackett-Burman method by Minitab statistical software was used to screen for effective factors such as initial uranium concentration, temperature, time, pH and amount of biomass on the removal of uranium by C. vulgaris. The results showed that the initial uranium concentration and pH factors were statistically effective by software. Optimization of effective factors in uranium bioremediation was evaluated by the response surface methodology (RSM). To determine the main effects and interaction of factors affecting uranium removal by C. vulgaris, central composite design (CCD) was used. The experimental data were then processed and the equation was evaluated to match the experimental data, and then the optimal removal values were determined. Finally, the results showed that C. vulgaris in optimal conditions proposed by Design-Expert software can remove 99.63% of existing uranium from aqueous solutions containing 7.1 mg / l uranium with pH 4.3. | ||
کلیدواژهها [English] | ||
Bioremediation, Experimental design, Heavy metals, Microalgae, Uranium | ||
مراجع | ||
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