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بررسی برخی تغییرات فیزیولوژیکی و بیوشیمیایی در سه رقم انگور (Viis vinifera L.) در پاسخ به تنش خشکی | ||
| زیست شناسی کاربردی | ||
| مقاله 4، دوره 34، شماره 3 - شماره پیاپی 69، آبان 1400، صفحه 58-81 اصل مقاله (580.92 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2021.31265.1370 | ||
| نویسندگان | ||
| اسحق شمسی پور1؛ محمد علی اعظمی* 2؛ محمد باقر حسن‌پور3 | ||
| 1دانشجوی کارشناسی ارشد گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه مراغه، مراغه | ||
| 2استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه مراغه، مراغه | ||
| 3پژوهشگر ارشد مرکز پژوهشهای مجلس شورای اسلامی | ||
| چکیده | ||
| جهت بررسی اثرات تنش خشکی بر سه رقم انگور (قزل اوزوم، خلیلی، پرلت) آزمایشی در قالب طرح کاملا تصادفی با چهار تکرار انجام گرفت. گیاهان انگور در چهار سطح تنش خشکی شامل شاهد، 40، 60 و 80 درصد ظرفیت زراعی رشد کردند. فاکتورهای مورفولوژیکی شامل وزن تر و وزن خشک گیاه و ویژگی های فیزیولوژیکی شامل محتوای پرولین، نشت الکترولیت، محتوای پراکسید هیدروژن و مالون دی آلدهید و فعالیت آنزیمی کاتالاز، آسکوربات پراکسیداز و گایاکول پراکسیداز مورد بررسی قرار گرفتند که سطوح مختلف معنی داری را نشان دادند. بیشترین میزان نشت الکترولیت و پراکسید هیدروژن در رقم خلیلی و سطح 40% تنش خشکی بود. رقم قیزیل اوزوم در تنش 60% بالاترین میزان پروتئین، آنزیم آسکوربات پراکسیداز، کاتالاز، گایاکول پراکسیداز و کمترین محتوای مالون دی آلدهید را نشان داد. در بررسی پارامترهای فلورسانس کلروفیل شامل فلورسانس حداقل (Fo)، فلورسانس متغیر (Fv)، فلورسانس حداکثر (Fm)، کارایی فتوشیمیایی موثر (YII)، حداکثر کارایی فتوسیستم II (Fv/fm)، کارایی کوانتومی غیرفتوشیمیایی تنظیم نشده فتوسیستم IIو (NO)Y تا سطح 60% تنش خشکی روند افزایش معنی داری در رقم قیزیل اوزوم به تنش خشکی مشاهده گردید. | ||
| کلیدواژه‌ها | ||
| آنزیم های آنتی اکسیدان؛ انگور؛ تنش خشکی؛ پرولین؛ فلورسانس کلروفیل | ||
| عنوان مقاله [English] | ||
| Evaluation some of the physiological and biochemical changes in three grapevine cultivars (Vitis vinifera L.) in response to drought stress | ||
| نویسندگان [English] | ||
| Eshagh shamsinow1؛ Mohammad Ali Aazami2؛ Mohamad Bagher Hasanpoor3 | ||
| 1M.SC.Gharwa Uloom Baghbani, Daneshgah Kashawarzi, Daneshgah Maragheh, Maragheh | ||
| 2Assistant Professor, Department of Horticultural Sciences, Faculty of Agriculture, Maragheh University, Maragheh | ||
| 3Senior Researcher of Islamic Council Research Center | ||
| چکیده [English] | ||
| In this study evaluate the effects of grapes cultivar on tolerance of vitis vinifera grown in drought stress conditions. Plants were grown in pots and exposed to 0, 40, 60 and 80 percent of fild capacity drought stress with three different cultivars (perlet, khaliliand ghizil ouzum). The physiological parameters such as MDA, H2O,EC and morphological paramaters such as dry and fresh weight and chlorophyll fluorescence Fo,Fv, Fm, Fv/Fm, Y(II) Y(NO) the contents of chlorophyll a, chlorophyll b, and enzyme activities of (APX, GPX, and CAT) and prolin content and protein percent were determined. Grapes cultivars positively (ghizil ouzum) affected on growth and physiology statistically. The highest, proline content was significantly different under drought stress condition and ghizil ouzum treatments so that, enzyme activities of (APX, GPX, and CAT) are increased with enhancement of drought levels .interaction effects of drought and cultivars in CAT, APX and GPX activity were significantly different compared with control and increased with enhancement of stress levels so that most of the activity was in 60% fc concentration and ghizil ouzum cultivar. The amount of chlorophyll fluorescence parameters (Fm, and Fv, Fv/Fm, Yll, Y(N0) were significant changes in drought stress treatment and ghizil ouzum in 60% FC compared with control. | ||
| کلیدواژه‌ها [English] | ||
| Antioxidant enzymes, Chlorophyll fluorescence, drought stress, grape, prolin | ||
| مراجع | ||
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