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اصلاح جهشی جهت افزایش توان تجمع کادمیوم در درختچه گز در مرحله کالوس | ||
زیست شناسی کاربردی | ||
دوره 33، شماره 1 - شماره پیاپی 63، اردیبهشت 1399، صفحه 128-147 اصل مقاله (662.52 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22051/jab.2019.19149.1211 | ||
نویسندگان | ||
مینا تقی زاده* 1؛ پروین کریمی2؛ موسی سلگی3 | ||
1استادیار .گروه علوم باغبانی- دانشکده کشاورزی و منابع طبیعی- دانشگاه اراک | ||
2کارشناسی ارشد . گروه علوم باغبانی- دانشکده کشاورزی و منابع طبیعی- دانشگاه اراک | ||
3استادیار . گروه باغبانی دانشگاه اراک | ||
چکیده | ||
گیاهپالایی بهعنوان روشی مقرون بهصرفه و سازگار با محیطزیست، در پاکسازی خاکهای آلوده به فلزات سنگین بهکار میرود. پژوهش حاضر با هدف بررسی مقاومت و پالایندگی کالوسهای درختچه گز تحت تاثیر القای جهش با استفاده از اتیل متان سولفونات انجام شد. در مرحله اول بهینه کردن القای کالوس و جهش در محیط کشت دارای غلظتهای مختلف 2,4- انجام شد. آزمایش سوم شامل ارزیابی میزان مقاومت و پالایش کالوس در غلظتهای صفر تا 40 میلیگرم در لیتر کادمیوم در دو نوع ریزنمونه تیمار شده و نشده با EMS بود. غلظت یک میلیگرم در لیتر 2,4-D بیشترین میزان زندهمانی و القای کالوس را در کمترین زمان ایجاد نمود. اعمال EMS با غلظت 2/0 درصد و زمان 30 دقیقه بیشترین میزان زندهمانی و کمترین میزان سیاه شدن ریزنمونهها را ایجاد کرد. بیشترین میزان تجمع کادمیوم بهمیزان 56/1053 میلیگرم در کیلوگرم ماده خشک کالوس در غلظت 40 میلیگرم در لیتر کادمیوم بهدست آمد. | ||
کلیدواژهها | ||
اتیل متان سولفونات؛ جهشزایی؛ فلزات سنگین؛ کشت بافت؛ گیاهپالایی | ||
عنوان مقاله [English] | ||
In vitro mutation breeding in Tamarix aphylla to increased Cd tolerance and accumulation | ||
نویسندگان [English] | ||
Mina Taghizadeh1؛ Parvin Karimi2؛ mousa solgi3 | ||
1Assistant Professor.department horticulture, arak university | ||
2Msc.department horticulture, arak university | ||
3Assistant Professor.Department of Horticulture, Arak University | ||
چکیده [English] | ||
Phytoremediation as a cost-effective and environmental friendly technique used to polluted soils with heavy metals contamination. The present study was aimed to evaluate the resistance and remediation of Tamarix aphylla calli affected by in vitro mutagenesis with. In the first step, optimization of callus induction and mutation in media with different concentrations of -2,4 was performed. The third experiments involved the evaluating of resistance and remeditaion rate of callus to cadmium at the concentrations of 0-40 mg L-1 in both treated and untreated explants with Ethyl methanesulfonate in medium. The highest survival and induction of callus was induced by the concentration of 1 mg L-1 2,4-D during the short time. Ethyl methanesulfonate at concentration of 0.2 percentage and 30 minutes made the maximum survival and minimum blacking phenomenon of explants. The highest accumulation of cadmium was obtained 1053.56 mg kg-1 dry weight in callus treated by 40 mg L-1 cadmium. | ||
کلیدواژهها [English] | ||
EMS, Mutation, Heaevy metals, Phytoremediation, Tissue culture | ||
مراجع | ||
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