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تاثیر کاربرد کود مرغی حاوی انروفلوکساسین بر تنفس، بیوماس میکروبی و جمعیت برخی باکتری‌های مفید خاک | ||
| زیست شناسی کاربردی | ||
| مقاله 9، دوره 32، شماره 4 - شماره پیاپی 62، اسفند 1398، صفحه 144-163 اصل مقاله (606.4 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2019.20737.1234 | ||
| نویسندگان | ||
| مهسا محمدزاده1؛ فروزان قاسمیان رودسری2؛ اکبر حسنی* 3؛ عباسعلی زمانی4 | ||
| 1گروه علوم محیط زیست، دانشکده علوم، دانشگاه زنجان | ||
| 2دانشگاه زنجان،دانشکده علوم، گروه زیست شناسی | ||
| 3گروه علوم خاک، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران | ||
| 4دانشگاه زنجان، دانشکده علوم، گروه علوم محیط زیست | ||
| چکیده | ||
| غلظت آنتیبیوتیکها در خاک بر اثر اضافه کردن کود مرغی آلوده به آنتیبیوتیک افزایش مییابد و ممکن است جامعه میکروبی خاک را تحت تاثیر قرار دهد. هدف این پژوهش تاثیر اضافه نمودن کود مرغی محتوی انروفلوکساسین به خاک بر جمعیتAzotobacter spp.، Pseudomonas fluorescens و Pseudomonas putida و همچنین بیوماس میکروبی و تنفس میکروارگانیسمهای خاک است. یک آزمایش در آزمایشگاه به مدت 45 روز با 7 تیمار و سه تکرار انجام شد. این آزمایش شامل کود مرغی تازه و کمپوست شده محتوی آنتیبیوتیک دردو سطح غلظتی بالا ( mg kg-12/26) و پایین (mg kg-123/4) و کود مرغی بدون آنتیبیوتیک بر مبنای طرح کرتهای به‌طورکامل تصادفی انجام شد. نتایج نشان داد کاربرد کود مرغی حاوی آنتیبیوتیک منجر به کاهش جمعیت Azotobacter spp.(%28)، Pseudomonas fluorescens(%73) و Pseudomonas putida(%6/14) در خاکها شد. کمپوست کردن کود مرغی نیز اگرچه اثر منفی آن را کمتر کرد اما همچنان کاهش جمعیت وجود داشت. کاربرد کود مرغی تازه بدون آنتی بیوتیک سبب افزایش 127 درصدی تنفس میکروبی خاک نسبت به شاهد شد اما بین سایر تیمارهای مختلف کاربرد کود مرغی تفاوت معنیدار دیده نشد. کاربرد کود مرغی بدون آنتیبیوتیک کربن بیوماس میکروبی را به طور معنیداری افزایش داد (%95). وجود آنتیبیوتیک در کود مرغی تاثیر منفی بر غلظت کربن بیوماس میکروبی داشت. به طور کلی رهاسازی و انتشار آنتیبیوتیکها در خاک بر اثر افزودن کود مرغی به مزارع حتی اگر قبل از مصرف کمپوست شده باشد، باعث کاهش رشد گروههای باکتریایی مفید خاک شد. | ||
| کلیدواژه‌ها | ||
| Azotobacter؛ Pseudomonas putida؛ Pseudomonas fluorescens؛ کمپوست؛ کود مرغی | ||
| عنوان مقاله [English] | ||
| Effect of chicken manure containing enrofloxacin on soil respiration, microbial biomass, and population of some beneficial bacteria | ||
| نویسندگان [English] | ||
| Mahsa Mohammadzadeh1؛ forouzan Ghasemian Roudsari2؛ Akbar Hassani3؛ Abbasali Zamani4 | ||
| 1Department of Envirnmental Science, Faculty of Science, University of Zanjan, Zanjan, I. R. Iran | ||
| 2Department of Biology,Faculty of Science, University of Zanjan, Zanjan, Iran, | ||
| 3Soil Science Department, Faculty of agriculture, University of zanjan, Zanjan, Iran | ||
| 4Department of Envirnmental Science, Faculty of Science, University of Zanjan, Zanjan, I. R. Iran | ||
| چکیده [English] | ||
| The concentration of antibiotics in the soil increases with the addition of fresh or composed chicken manure containing antibiotics and may affect the microbial community of the soil. The purpose of this study was to evaluate the effect of adding enrofloxacin containing chicken manure to soil on the population of Azotobacter spp, Pseudomonas putida and Pseudomonas fluorescens, as well as microbial biomass and respiration of soil. An experiment was conducted in a laboratory for 45 days with 7 treatments and three replications including fresh and composted chicken manure containing enrofloxacin in two levels of high (26.2 mg kg-1) and low (4.23 mg kg-1) concentrations and manure without any antibiotic based on a completely randomized design. The results showed that the application of chicken manure containing enrofloxacin reduced the population of Azotobacter spp (28 percent), Pseudomonas fluorescens (73 percent) and Pseudomonas putida (14.6 percent) in the soils. However, the composting of chicken manure, although reducing its negative effect, still led to a decline in population. Application of fresh chicken manure led to a 127 percent increase in microbial respiration of the soil compared to control but between other treatments there was no meaningful difference. The use of chicken manure without antibiotic significantly increased the microbial biomass (95 percent). The presence of high concentration of enrofloxacin in poultry manure had a negative effect on the microbial biomass increase, especially at the beginning of the period. | ||
| کلیدواژه‌ها [English] | ||
| Azotobacter, Compost, Pseudomonas fluorescens, Pseudomonas putida | ||
| مراجع | ||
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Anderson, J. and Domsch, K. (1978) Mineralization of bacteria and fungi in chloroform-fumigated soils. Soil Biology and Biochemistry 10:207-213. Čermák, L., Kopecký, J., Novotná, J., Omelka, M., Parkhomenko, N., Plháčková, K. and Ságová-Marečková, M. (2008) Bacterial communities of two contrasting soils reacted differently to lincomycin treatment. Applied Soil Ecology 40:348-358. Colinas, C., Ingham, E. and Molina, R. (1994) Population responses of target and non-target forest soil organisms to selected biocides. Soil Biology and Biochemistry 26:41-47. Cordova-Kreylos, A.L. and Scow, K.M. (2007) Effects of ciprofloxacin on salt marsh sediment microbial communities. The ISME Journal 1:585. Cui, H., Wang, S.P., Fu, J., Zhou, Z.Q., Zhang, N. and Guo, L. (2014) Influence of ciprofloxacin on microbial community structure and function in soils. Biology and Fertility of Soils 50:939-947. Davis, K.E., Joseph, S.J. and Janssen, P.H. (2005) Effects of growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria. Applied and Environmental Microbiology 71:826-834. Diao, X., Sun, Y., Sun, Z. and Shen, J. (2004). Effects of Apramycin on microbial activity in different types of soil. Ecology and Environment 13:565-568. Ding, C. and He, J. (2010) Effect of antibiotics in the environment on microbial populations. Applied Microbiology and Biotechnology 87:925-941. Ding, G.C., Radl, V., Schloter-Hai, B., Jechalke, S., Heuer, H., Smalla, K. and Schloter, M. (2014) Dynamics of soil bacterial communities in response to repeated application of manure containing sulfadiazine. PLoS One 9:e92958. Fründ, H.C., Schlösser, A. and Westendarp, H. (2000) Effects of tetracycline on the soil microflora determined with microtiter plates and respiration measurement. Mitteilgn. Dtsch. Bodenkundl. Gesellsch 93:244-247. Grenni, P., Ancona, V. and Caracciolo, A.B. (2018) Ecological effects of antibiotics on natural ecosystems: A review. Microchemical Journal 136:25-39. Hammesfahr, U., Heuer, H., Manzke, B., Smalla, K. and Thiele-Bruhn, S. (2008) Impact of the antibiotic sulfadiazine and pig manure on the microbial community structure in agricultural soils. Soil Biology and Biochemistry 40:1583-1591. Hayat, R., Ali, S., Amara, U., Khalid, R. and Ahmed, I. (2010). Soil beneficial bacteria and their role in plant growth promotion: a review. Annals of Microbiology, 60(4): 579-598 Hund-Rinke, K., Simon, M. and Lukow, T. (2004) Effects of tetracycline on the soil microflora: function, diversity, resistance. Journal of Soils and Sediments 4:11. Katoh, K. and Itoh, K. (1983) New selective media for Pseudomonas strains producing fluorescent pigment. Soil Science and Plant Nutrition 29:525-532. Kim, K.R., Owens, G., Kwon, S.I., So, K.H., Lee, D.B. and Ok, Y.S. (2011) Occurrence and environmental fate of veterinary antibiotics in the terrestrial environment. Water, Air, & Soil Pollution 214:163-174. King, E. O., Ward, M. K., and Raney, D. E. (1954) Two simple media for the demonstration of pyocyanin and fluorescin. Translational Research, 44(2): 301-307. Kotzerke, A., Hammesfahr, U., Kleineidam, K., Lamshöft, M., Thiele-Bruhn, S., Schloter, M. and Wilke, B.M. (2011) Influence of difloxacin-contaminated manure on microbial community structure and function in soils. Biology and Fertility of Soils 47:177-186. Kotzerke, A., Sharma, S., Schauss, K., Heuer, H., Thiele-Bruhn, S., Smalla, K., Wilke, B.M. and Schloter, M. (2008) Alterations in soil microbial activity and N-transformation processes due to sulfadiazine loads in pig-manure. Environmental Pollution 153:315-322. Kuhlmann, J., Dalhoff, A. and Zeiler, H.J. (2012) Quinolone antibacterials. Springer Science & Business Media Lewis, K. (2013) Platforms for antibiotic discovery. Nature reviews Drug discovery 12: 371. Liu, F., Ying, G.G., Tao, R., Zhao, J.L., Yang, J.F. and Zhao, L.F. (2009) Effects of six selected antibiotics on plant growth and soil microbial and enzymatic activities. Environmental Pollution 157:1636-1642. Liu, H., Pu, C., Yu, X., Sun, Y. and Chen J. (2018) Removal of tetracyclines, sulfonamides, and quinolones by industrial-scale composting and anaerobic digestion processes. Environmental Science and Pollution Research:1-10. Näslund, J., Hedman, J.E. and Agestrand, C. (2008) Effects of the antibiotic ciprofloxacin on the bacterial community structure and degradation of pyrene in marine sediment. Aquatic Toxicology 90:223-227. Poulsen, P.H., Al-Soud, W.A., Bergmark, L., Magid, J., Hansen, L.H. and Sørensen, S.J. (2013) Effects of fertilization with urban and agricultural organic wastes in a field trial–Prokaryotic diversity investigated by pyrosequencing. Soil Biology and Biochemistry 57:784-793. Slana, M., Pahor, V., Cvitkovič Maričič, L. and Sollner‐Dolenc, M. (2014) Excretion pattern of enrofloxacin after oral treatment of chicken broilers. Journal of Veterinary Pharmacology and Therapeutics 37:611-614. Slana, M. and Sollner-Dolenc, M. (2016) Enrofloxacin degradation in broiler chicken manure under various laboratory conditions. Environmental Science and Pollution Research 23:4422-4429. Slana, M., Žigon, D. and Sollner-Dolenc, M. (2017) Enrofloxacin degradation in broiler chicken manure under field conditions and its residuals effects to the environment. Environmental Science and Pollution Research 24:1-10. Thiele-Bruhn, S. and Beck, I.C. (2005) Effects of sulfonamide and tetracycline antibiotics on soil microbial activity and microbial biomass. Chemosphere 59:457-465. Thiele‐Bruhn, S. (2003) Pharmaceutical antibiotic compounds in soils–a review. Journal of Plant Nutrition and Soil Science 166:145-167. Vaclavik, E., Halling-Sørensen, B. and Ingerslev, F. (2004) Evaluation of manometric respiration tests to assess the effects of veterinary antibiotics in soil. Chemosphere 56:667-676. Vance, E.D., Brookes, P.C. and Jenkinson, D.S. (1987) An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry 19:703-707. Wei, Z., Wang, J., Zhu, L., Wang, J., and Zhu, G. (2018) Toxicity of enrofloxacin, copper and their interactions on soil microbial populations and ammonia-oxidizing archaea and bacteria. Scientific reports, 8(1); 5828 Westergaard, K., Müller, A., Christensen, S., Bloem, J and, Sørensen, S. (2001) Effects of tylosin as a disturbance on the soil microbial community. Soil Biology and Biochemistry 33:2061-2071. Zarfl, C., Klasmeier, J. and Matthies, M. (2009) A conceptual model describing the fate of sulfadiazine and its metabolites observed in manure-amended soils. Chemosphere 77:720-726. Zhou, X., Chen, C., Yue, L., Sun, Y., Ding, H. and Liu, Y. (2008) Excretion of enrofloxacin in pigs and its effect on ecological environment. Environmental Toxicology and Pharmacology 26:272-277. Zielezny, Y., Groeneweg, J., Vereecken, H. and Tappe, W. (2006). Impact of sulfadiazine and chlorotetracycline on soil bacterial community structure and respiratory activity. Soil Biology and Biochemistry 38:2372-2380.
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