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اثر نانو ذرات نقره بر ظرفیت آنتی اکسیدان و الگوی پروتئین محلول کل در گیاهچههای گوجه در شرایط این ویترو | ||
زیست شناسی کاربردی | ||
مقاله 2، دوره 32، شماره 3 - شماره پیاپی 61، آذر 1398، صفحه 22-38 اصل مقاله (988.39 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22051/jab.2020.4405 | ||
نویسنده | ||
رویا رضوی زاده* | ||
دانشیار، گروه زیست شناسی، دانشگاه پیام نور، صندوق پستی3697-19395، تهران- ایران | ||
چکیده | ||
در این مطالعه تاثیر نانو ذرات نقره در اندازههای بین 94/53 و 5/288 نانومتر و در غلظتهای 0، 5/2، 5، 10، 20، 40، 80 و 100 پیپیام بر روی تغییرات شاخص های فیزیویولوژیکی مانند آنتوسیانین کل، الگوی پروتئین محلول کل و فعالیت آنزیمهای کاتالاز، آسکوربات پراکسیداز و سوپراکسید دیسموتاز گیاهچههای گوجه (Solanum Lycopersicon)در محیط کشت درون شیشه مورد بررسی قرارگرفت. نتایج نشان داد که میزان آنتوسیانین در پاسخ به حضور نانوذرات نقره کاهش معنیداری را تا غلظت 10 پیپیام به دنبال داشته است. پروتئین محلول کل گیاه با افزایش غلظت نانوذره در اندام هوایی افزایش و در ریشه با افزایش غلظت در 20 و 40 پی پی ام نسبت به شاهد کاهش نشان داد. همچنین شدت بیان شش باند پروتئینی تحت تیمار با نانو ذرات نقره تغییر نمود.با افزایش غلظت نانو ذرات در محیط کشت افزایش در فعالیت آنزیمهای آنتی اکسیدانت در ریشهها و اندام هوایی مشاهده گردید. | ||
کلیدواژهها | ||
آنزیمهای آنتی اکسیدان؛ پروتئین محلول کل؛ نانو فناوری؛ نانو ذرات نقره | ||
عنوان مقاله [English] | ||
The effect of silver nanoparticles on the antioxidant capacity and total soluble protein pattern in tomato seedlings under in vitro culture | ||
نویسندگان [English] | ||
Roya Razavizadeh | ||
Department of Biology, Payame Noor University, PO BOX 19395-3697 Tehran, Iran. | ||
چکیده [English] | ||
TodayToday, synthetic nanoparticles encompass a wide range of particles with unique properties and have many applications in the field of nanotechnology. Due to the emergence and lack of understanding of the consequences of using nanoparticles, investigating the effect of releasing these particles in the environment at various biological levels is important. In this study, the effects of nanoparticles of silver in concentrations of 0, 2.5, 5, 10, 20, 40, 80 and 100 ppm on physiological parameters such as total anthocyanin, total soluble protein and enzyme activities of Catalase, Ascorbate peroxidase and Superoxide dismutase in tomato seedlings (Solanum Lycopersicon) under in vitro were evaluated. The results showed that anthocyanin levels in response to the presence of silver nanoparticles decreased significantly to 10 ppm concentration. The total protein soluble protein increased with increase in the concentration of nanoparticles in the shoot and decreased with increasing concentrations at 20 and 40 ppm compared to the control. The intensity of expression of six protein bands treated with silver nanoparticles also changed. Increasing the concentration of nanoparticles in the medium increased the activities of catalase and superoxide dismutase and decreased ascorbate peroxidase activity in the roots. However, in shoot, increased activity of peroxidase and catalase, and decreased activity of superoxide dismutase in response to high concentrations of silver nanoparticles. The results indicated that the presence of silver nanoparticles in the tomato culture medium leads to responses at physiological and molecular levels. | ||
کلیدواژهها [English] | ||
Antioxidant enzymes, nanotechnology, silver nanoparticles, total soluble protein | ||
مراجع | ||
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