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بررسی پاسخهای دفاع آنتیاکسیدانی در سه ژنوتیپ Lolium perenne تحت تنش خشکی | ||
زیست شناسی کاربردی | ||
مقاله 4، دوره 32، شماره 1 - شماره پیاپی 59، خرداد 1398، صفحه 57-75 اصل مقاله (679.47 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22051/jab.2019.4308 | ||
نویسندگان | ||
فرزانه ترکش1؛ لیلا شبانی* 2؛ محمدرضا سبزعلیان1 | ||
1گروه زیست شناسی، دانشکده علوم، دانشگاه شهرکرد، شهرکرد | ||
2گروه علوم گیاهی، دانشکده علوم، دانشگاه شهرکرد، شهرکرد | ||
چکیده | ||
هدف پژوهش حاضر بررسی اثر تنش خشکی بر پاسخهای دفاعی آنتیاکسیدان در 3 ژنوتیپ از گیاه چچم و در قالب طرح کامل تصادفی با سه تکرار در شرایط مزرعهای انجام شد. تنش خشکی RWC، وزن تر و خشک، محتوای کلروفیل a، b و کاروتنوئید را کاهش داد، درحالیکه غلظت H2O2 و محتوای پرولین افزایش یافت. همچنین در مقایسه با شاهد، فعالیت آنزیمهای CAT،APX ، GPX و SOD به علت تنش خشکی افزایش یافت. باتوجه به نتایج به دست آمده شامل کاهش اندک محتوای نسبی آب، افزایش فعالیت آنزیم های آنتی اکسیدان و افزایش میزان پرولین در ژنوتیپ اول میتوان نتیجه گرفت که احتمالا این ژنوتیپ در مقایسه با دو ژنوتیپ دیگر نسبت به تنش خشکی دارای مقاومت بیشتری است. | ||
کلیدواژهها | ||
آنتی اکسیدان؛ پراکسید هیدروژن؛ پرولین؛ چچم؛ خشکی | ||
عنوان مقاله [English] | ||
urvey of antioxidant defensive responses in three genotypes of Lolium pernne under drought stress | ||
نویسندگان [English] | ||
Farzane Tarkesh1؛ Leila shabani2؛ Mohammad R. Sabzalian1 | ||
1Department of Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran | ||
2Department of Plant Science, Faculty of Science, Shahrekord University, Shahrekord, Iran | ||
چکیده [English] | ||
The present study was aimed to investigate the effect of drought stress on antioxidant defensive responses of three genotypes of Lolium perenne. This investigation was performed as factorial and a randomized complete design with three replications under field conditions. The results indicated that drought stress significantly decreased the relative water content (RWC), fresh and dry weight, chlorophyll a, chlorophyll b and carotenoid contents, while the concentration of H2O2 and proline increased. Also, compared to the control, the activities of catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and superoxide dismutase (SOD) increased due to drought stress. Regarding results of the relative water content, the activity of antioxidant enzymes and proline content, it is concluded that genotype 1 had more tolerance to drought compared to the two other genotypes. | ||
کلیدواژهها [English] | ||
Antioxidant, Drought, H2O2, Lolium, Proline | ||
مراجع | ||
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