پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 25 |
| تعداد شماره‌ها | 939 |
| تعداد مقالات | 7,734 |
| تعداد مشاهده مقاله | 12,722,377 |
| تعداد دریافت فایل اصل مقاله | 9,063,999 |
تولید زایلیتول توسط سویه مخمری دباریومایسس هانسنی جداسازی شده از گرده گل پنیرک | ||
| زیست شناسی کاربردی | ||
| مقاله 11، دوره 31، شماره 1 - شماره پیاپی 55، خرداد 1397، صفحه 164-177 اصل مقاله (1.1 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2017.4295. | ||
| نویسندگان | ||
| فروغ عسگری کرچگانی* 1؛ مرضیه شمعی1؛ غلامرضا قزلباش2؛ ایرج نحوی3 | ||
| 1دانشجوی کارشناسی ارشد میکروبیولوژی، دانشگاه آزاد اسلامی واحد فلاورجان. | ||
| 2استادیار میکروبیولوژی، دانشگاه شهید چمران اهواز، دانشکده علوم، گروه زیست شناسی. | ||
| 3استاد، دانشگاه اصفهان، دانشکده علوم، گروه زیست شناسی. | ||
| چکیده | ||
| زایلیتول یک پلی‌اُول پنج‌کربنه با قدرت شیرین‌کنندگی بالاست. این پلی‌اُول شیرین‌ترین پلی‌الکل می‌باشد. تولید بیوتکنولوژیکی زایلیتول با استفاده از میکروارگانیسم‌ها از نظر زیست محیطی ایمن است و به عنوان یک روش جایگزین روش شیمیایی مورد مطالعه قرار می‌گیرد. با توجه به خواص ارزشمندی که زایلیتول دارد به عنوان جایگزین قند در صنایع شیمیایی، غذایی و دارویی استفاده می‌شود. بر اساس مطالعات اخیر، در میان میکروارگانیسم‌ها، مخمرها بهترین تولیدکننده می‌باشند. در این پژوهش این شیرین‌کننده ارزشمند توسط سویه مخمری دباریومایسس هانسنی تولید شد. این مخمر از گرده گل پنیرک جداسازی و با تعیین توالی 18S rRNA و هم ردیفی با توالی های موجود در پایگاه داده ها شناسایی گردید. زایلیتول در محیط تولید حاوی زایلوز و دیگر مواد معدنی تولید شد و با روش های آنالیز کروماتوگرافی لایه نازک و با استفاده از کیت مگازایم ایرلند شناسایی و تایید و مقدار آن با روش کروماتوگرافی مایع با عملکرد بالا اندازه‌گیری گردید. سویه مخمری دباریومایسس هانسنی قادر به تولید 45/20 گرم بر لیتر زایلیتول از 40 گرم بر لیتر زایلوز، بعد از 48 ساعت بود. | ||
| کلیدواژه‌ها | ||
| زایلیتول؛ زایلوز؛ Dataryum Hansen | ||
| عنوان مقاله [English] | ||
| Production of xylitol by Debaryomyces hansenii , the yeast strain isolated from pollen of Malva sylvestris flower | ||
| نویسندگان [English] | ||
| Forough Asgary Karchegany1؛ Marzieh Shamee1؛ Gholamreza Ghezelbash2؛ Iraj Nahvi3 | ||
| 1MSc of Microbiology, Falavarjan Azad University, Isfahan, Iran. | ||
| 2Assistant Professor, Department of Biology, Faculty of Science, Shahid Chamran , Ahvaz, Iran. | ||
| 3Professor, Department of Biology, Faculty of Science, Isfahan University, Isfahan, Iran. | ||
| چکیده [English] | ||
| Xylitol is a naturally five – carbon polyol with a high sweetening power. Xylitol is the sweetest among the polyalcohols. The biotechnological method of producing xylitol by microorganisms has been studied as an alternative to the chemical method and is very environmentally safe. Xylitol is an attractive sugar substitute for use in the food-processing, nutritional supplement and pharmaceutical industries. Among the microorganisms, yeasts are considered as the best xylitol producer. The purpose of this study was xylitol production by Debaryomyces hansenii that isolated from pollen of Malva sylvestris flower. This strain was identified by 18SrRNA sequencing and following its alignment with existed similar strains in data bases. Xylitol was produced in production medium contained xylose and other compounds. Xylitol was first identified by thin layer chromatography (TLC) method then it was confirmed by Megasyme kit. Xylitol concentration was quantified using high performance liquid chromatography ( HPLC) method. This strain produced 20.45 g l-1 xylitol after 48 hours culture incubation in medium contained 40 g l-1 xylose. | ||
| کلیدواژه‌ها [English] | ||
| Debaryomyces hansenii, xylitol, xylose | ||
| مراجع | ||
|
Abou Zeid, A. A., El-Fouly, M. Z., El-Zawahry, Y. A., El-Mongy, T.M., El-Aziz, A.B.A. (2008) Bioconversion of rice straw xylose to xylitol by a local strain of candida tropicalis Journal of Applied Sciences Research 4(8): 975-986. Ahmad, I., Shim, W.Y., Ieon, W.Y., Yoon, B.H., Kim, J.H. (2012) Enhancement of xylitol production in Candida tropicalis by co-expression of two genes involved in pentose phosphate pathway Bioprocess and Biosystes Engineering 35(1-2):199-04. Altamirano, A., Vazquez, F., De Figueroa, L.I.C. (2000) Isolation and identification of xylitol- producing yeasts from agricultural residues Folia Microbiological 45(3): 255-258. Branco, R.F., Santos, J.C.D., Sarrouh, B.F., Rivaldi, J.D., Jr, A.P., Silva, S.S.D. (2009) Profiles of xylose reductase, xylitol dehydrogenase and xylitol production under different oxygen transfer volumetric coefficient values Journal of Chemical Technology and Biotechnology 84: 326-330. Bura, R., Vajzovic, A., Doty, S.L. (2012) Novel endophytic yeast Rhodotorula mucilaginosa strain PTD3I: production of xylitol and ethanol Journal of Industrial Microbiology and Biotechnology 39(7): 1003-1011. Carvalheiro, F., Duarte, L.C., Lopes, S., parajo, J.C., Pereira, H., Girio, F.M. (2006) Supplementation requirements of brewery’s spent grain hydrolysate for biomass and xylitol production by Debaryomyces hansenii CCMI 941 Journal of Microbiology Biotechnology 33: 646- 654. Cheng, k.k., Ling, H.Z., Zhang, J.A., Ping, W.X., Huang, W., Ge, J.P., Xu, J.M. (2010) Strain isolation and study on process parameters for xylose- to- Xylitol bioconversion Food Biotechnology 24(1): 1606- 1611. Cortez, D.V., Roberto, I.C. (2014) Optimization of D-xylose to xylitol biotransformation by Candida guilliermondii cells permeabilized with Triton x-100 Biocatalysis and Biotrasformation 32(1): 34-38. Deak, T., Chen, J., Beuchat, L.R. (2000) Molecular characterization of Yarrowia lipolytica and Candida zeylanoides isolated from poultry Applied and Environental Microbiology 66: 4340-4344. El-Batal, A.I., Khalaf, S.A. (2004) Xylitol production from corn corbs hemicellulosic hydrolysate by Candida tropicalis immobilized cells in hydrogel copolymer carrier Journal of Agricultural Biological 1066-1073. Granstrom, T.B., Izumori, K., Leisola, M. (2007) A rare sugar xylitol PartI: the biochemistry and biosynthesis of xylitol Applied Microbiology Biotechnology 2: 277-281. Hoffman, CS (1997) Current Protocols in Molecular Biology John Wiley and Sons,New York. Ko, C.H., Chiang, P.N., Chiu, P.C., Liu, C.C., Yang, C.L., Shiau, I.L. (2008) Integrated xylitol production by fermentation of hardwood wastes Journal of Chemical Technology Biotechnology 83: 534-540. Li, Y., Park, J.Y., Shiroma, R., Ike, M., Tokuyasu, K. (2012) Improved ethanol and reduced xylitol production from glucose and xylose mixtures by the mutant strain of Candida shehatae ATCC22984 Applied Biochemistry Biotechnology 166:1781- 1790. LifHolgerson, P., Stecksen Blicks, C., Sjostrom, I., Oberg, M., Twetman, S. (2006) Xylitol concentration in saliva and dental plaque after use of various xylitol-contain ing products. Caries Research 40: 393-397. Misra, S., Raghuwanshi, S., Saxena, R.K. (2012) Fermentation behavior of an osmotolerant yeast Debaryomyces hansenii for xylitol production Biotechnology Progress 28(6): 1457-1465. Natah, S.S., Hussien, K.R., Tuominen, J.A., Koivisto, V.A. (1997) Metabolic response to lactitol and xylitol in healthy men Journal of Clinical Nutrition 65: 947-950. Onishi, H., Suzuki, T (1969) Microbial production of xylitol from glucose Applied Microbiology18: 1031-1035. Perez-Bibbins, B., Salgado, J.M., Torrado, A., Aguilar-Uscanga, M.G., Dominguez, J.M. (2013) Culture parameters affecting xylitol production by Debaryomyces hansenii immobilized in alginate beads Process Biochemistry 48(3): 387-397. Perez-Bibbins, B., Oliveira, R.R.S., Torrado, A., Aguilar-Uscanga, M.G., Dominguez, J.M. (2014) Study of the potential of the air lift bioreactor for xylitol production in fed-boten cultures by Debaryomyces hansenii immobilized in alginate beads- Biotechnol. Process Engineering 98: 151-161. Phaff, H.J., Starmer, W.T. (1987) Yeast associated with plants, insect and soil Pp. 123-180. In: Rose, A.H.and Harrison, J.S. (eds). The yeasts: Biology of yeasts second edition, Academic Press, Orlando, Florida. Ping, y., Ling, H.Z., Song, G., Ge, J.P. (2013) Xylitol production from non-detoxified corncob hemicellulose acid hydrolysate by Candida tropicalis Biochemical Engineering Journal 75:89-91. Prakasha, G., Varma, A.J., Prabhune, A., Shouche, Y., Rao, M. (2011) Microbial production of xylitol from D-xylose and sugarcane bagasse hemicellulose using newly isolated thermotolerant yeast Debaryomyces hansenii Bioresource Technology 102(3): 3304-3308. Ramesh, S., Muthuvelayudham, R., Rajesh Kannan, R., Viruthagiri, T. (2012) Application of factorial design to the study of xylitol production from corncob hemicellulose hydrolysate by Candida guilliermondii Journal of Biochemical and Technology 4(1): 518-523. Ramesh, S., Muthuvelayudham, R., Kannan, R.R., Viruthagiri, T. (2013) Stastical opti mization of process variables for corncob hemicellulose hydrolysate to xylitol by Debaryomyces hansenii var hansenii International Journal of Chemical and Technology 5(1): 186-196. Rao, R.S., Bhadra, B., Shivaji, S. (2007) Isolation and characterization of xylitol- producing yeasts from the gut of colleopteran insects Current Microbiology 5:441-446. Rao, R.S., Prahasham, R.S., Prasad, K.K., Rajesham, S., Sarma, P.N., Rao, L.V. (2004) Xylitol production by Candida sp.: Parameter optimization using Taguchi approach .Process Biochemistry 39: 951- 956. Saha, B.C., Bothast, R.J. (1999) Production of xylitol by Candida peltata Journal of Industrial Microbiology and Biotechnology 22: 633- 636. Sampaio, F.C., Chaves-Alves, V.M., Converti, A., Lopes Passos, F.M., Cavalcante Coelho, J.L. (2008) Influence of cultivation conditions on xylose-to xylitol bioconversion by a new isolate of Debaryomyces hansenii Bioresource Technology 99: 502-508. Sirisansaneeyakul, S., Kop, B., Tochampa, W., Wannawilai, S., Chaveesuk, R., Lee, W.C. (2014) Sodium benzoate stimulates xylitol production by Candida mogii Journal of Institute Chemical Engineering 45(3): 734-743. Toyoda, T., Ohtaguchi, K. (2009) Xylitol production from lactose by biotransformation Journal of Biochemical Technology 2: 126-132. Vakhlu, J., Kour, A. (2006) Yeast lipases: enzyme purification, biochemical properties and gene cloning Electronic Journal of Biotechnology 9: 69-85. Zagustina, N.A., Rodionova, N.A., Mestechkina, N.M., Shcherbukhin, V.D., Bezborodov, A.M. (2001) Xylitol production by a culture of Candida guilliermondii 2581 Applied Microbiology Biotechnology 37(5):489-492. Zhang, J., Geng, A., Yoo, C., Lu, Y., Li, Q. (2012) Xylitol production from D-xylose and horticultural waste hemicellulosic hydrolysate by a new isolate of Candida athensensis SB18 Bioresource Technology 105: 134-141. | ||
|
آمار تعداد مشاهده مقاله: 466 تعداد دریافت فایل اصل مقاله: 626 |
||