پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 25 |
| تعداد شماره‌ها | 967 |
| تعداد مقالات | 8,010 |
| تعداد مشاهده مقاله | 13,573,664 |
| تعداد دریافت فایل اصل مقاله | 9,616,332 |
تاثیر گونه‌های مختلف میکوریز بر خصوصیات رشدی، ترکیبات فنلی و فعالیت آنتی اکسیدانی گیاه پونهسای بینالودی (Nepeta Binaludensis) | ||
| زیست شناسی کاربردی | ||
| مقاله 2، دوره 37، شماره 3 - شماره پیاپی 81، آذر 1403، صفحه 11-22 اصل مقاله (835.53 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2024.43026.1557 | ||
| نویسندگان | ||
| علی گنجعلی* 1؛ نسرین رجبی2؛ فایزه بیات2 | ||
| 1استاد، گروه زیست شناسی-دانشکده علوم پایه-دانشگاه فردوسی مشهد | ||
| 2کارشناسی ارشد، گروه زیست شناسی- دانشکده علوم پایه- دانشگاه فردوسی مشهد | ||
| چکیده | ||
| مقدمه: گیاه پونهسای بینالودی با نام علمی Nepeta Binaludensis)) گونه‌ای چندساله و علفی از خانواده نعنائیان می‌باشد. این گیاه به دلیل وجود ترکیبات موثر در درمان بسیاری از بیماری‌ها مورد استفاده قرار می‌گیرد اما از سویی به دلیل افزایش برداشت گیاه از عرصه، در خطر انقراض قرار گرفته است پس باید راه‌کار مناسبی جهت افزایش زیست توده گیاه در عین حفظ این گونه گیاهی در نظر گرفته شود. مواد و روش: در این پژوهش تاثیر گونه‌های مختلف قارچ میکوریز شامل Glomus hoi ، Glomus interaradis و Glomus mossea بر خصوصیات رویشی و میزان ترکیبات شیمیایی پونه‌سا بینالودی مورد بررسی قرار گرفت. گلدان‌های یک کیلوگرمی حاوی خاک باغچه به عنوان واحد آزمایشی در نظر گرفته شدند. گیاهان در شرایط فیتوترون با دوره نوری 16 و 8 ساعت به ترتیب روشنایی و تاریکی به مدت 20 هفته در دمای 25 درجه سانتی گراد رشد کردند. نتایج و بحث: نتایج نشان داد میکوریز و بویژه گونه G. intraradices تاثیر معنی داری بر اغلب صفات مورفولوژیکی گیاه داشت. در این مطالعه بیشترین میزان فنل کل (mg/100gDW 91/296)، فلاونوئیدکل (mg/100gDW 5/33) و ظرفیت آنتی‌اکسیدانی به گیاهان تلقیح شده با گونه G. intraradices مربوط بود. همچنین انباشت عناصر غذایی مانند کلسیم، پتاسیم، آهن، منیزیم و فسفر در گیاهان میکوریزی به صورت معنی داری بیشتر از گیاهان شاهد بود. نتایج این بررسی موید آن است که استفاده از قارچ‌های میکوریز به عنوان کودهای بیولوژیک و سازگار با محیط زیست می‌تواند جایگزین مناسبی برای کودهای شیمیایی باشند. | ||
| کلیدواژه‌ها | ||
| پونه‌سا بینالودی؛ صفات مورفولوژیکی؛ فنل کل؛ قارچ میکوریز | ||
| عنوان مقاله [English] | ||
| The effect of mycorrhizal inoculation on growth characteristics, phenolic compounds and antioxidant activity of (Nepeta binaludensis Jamzad) | ||
| نویسندگان [English] | ||
| Ali Gangali1؛ Nassrin Rajabi2؛ Fayeze Bayat2 | ||
| 1Professor, Department of Biology, Faculty of Basic Sciences, Ferdowsi University of Mashhad | ||
| 2Master's degree, Department of Biology - Faculty of Basic Sciences - Ferdowsi University of Mashhad | ||
| چکیده [English] | ||
| Introduction: Nepeta Binaludensis Jamzad is a perennial and herbaceous species of the Laminasea family. Limited distribution, indiscriminate harvesting and destruction of natural habitats have put this plant in danger of extinction. In this research, the effect of different species of mycorrhiza fungi including: Glomus hoi, Glomus interaradis and Glomus mossea on the morphological and biochemical characteristics of N. binaludensis were investigated. Methods: In this experiment, 1Kg pots containing garden soil were considered as experimental units. Mycorrhiza species were added to three cm above the soil. The plants were grown for 20 weeks at 25° C and with a photoperiod of 16 / 8 hours of light/darkness. Results and discussion: The results showed that fungal species, especially G. intraradices, had considerably effect on most morphological characteristics. The highest content of total phenol, flavonoids and antioxidant capacity were recorded in plants inoculated with G. intraradices. Also, the accumulation of nutrients such as calcium, potassium, iron, magnesium and phosphorus in mycorrhizal plants was significantly higher than the control plants. The results of this study were confirmed that the use of mycorrhizal fungi as biological and environmentally friendly fertilizers can be a suitable alternative for chemical fertilizers. | ||
| کلیدواژه‌ها [English] | ||
| Nepeta Binaludensis, Morphological traits, Total phenol, Mycorrhiza fungi | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
Abbott, L. K., and Robson, A. D. (1985). Formation of external hyphae in soil by four species of vesicular‐arbuscular mycorrhiza al fungi. New Phytologist. 99(2): 245-255. Adeyemi, N. O., Atayese, M. O., Sakariyawo, O. S., Azeez, J. O., Olubode, A. A., Ridwan, M., Adebiyi, O., Oni, O., and Ibrahim, I. (2021). Influence of different arbuscular mycorrhizal fungi isolates in enhancing growth, phosphorus uptake and grain yield of soybean in a phosphorus deficient soil under field conditions. Communications in Soil Science and Plant Analysis. 52(10): 1171-1183. Allen, E.B. and Allen, M.F. (1986). Water relations of xeric grasses in the field: interactions of mycorrhizas and competition. New Phytologist. (104): 559-571. Ansori, A., and Gholami, A. (2015). Improved nutrient uptake and growth of maize in response to inoculation with Thiobacillus and mycorrhiza on an alkaline soil. Communications in Soil Science and Plant Analysis. 46(17): 2111-2126. Auge, R. M. (2001). Stomatal behavior of arbuscular mycorrhizal plants. In Arbuscular mycorrhizas: physiology and function. Springer, Dordrecht. 201-237. Barea, J. M., Azcon, R., and Azcon-Aguilar, C. (2005). Interactions between mycorrhizal fungi and bacteria to improve plant nutrient cycling and soil structure. In Microorganisms in soils: roles in genesis and functions. Springer, Berlin, Heidelberg. 195-212. Begum, N., Qin, C., Ahanger, M. A., Raza, S., Khan, M. I., Ashraf, M., Ahmed, N., and Zhang, L. (2019). Role of arbuscular mycorrhizal fungi in plant growth regulation: implications in abiotic stress tolerance. Frontiers in plant science. 10: 1068. Blilou, I., Ocampo, J.A., and Garcia-Garrido, J.M. (2000). Induction of LTP (Lipid transfer protein) and PAL (phenylalanine ammonia-lyase) gene expression in rice roots colonized by the arbuscular mycorrhizal fungus Glomus mosseae. Journal of experimental botany. 51(353): 1969-1977. Bonfante, P., and Genre, A. (2010). Mechanisms underlying beneficial plant-fungus interactions in mycorrhizal symbiosis. Nature communications. 1(1): 48. Caravaca, F., Diaz, E., Barea, J. M., Azcón-Aguilar, C., and Roldan, A. (2003). Photosynthetic and transpiration rates of Olea europaea subsp. sylvestris and Rhamnus lycioides as affected by water deficit and mycorrhiza. Biologia Plantarum. 46(4): 637-639. Cardoso, I. M., and Kuyper, T. W. (2006). Mycorrhizas and tropical soil fertility. Agriculture, Ecosystems and Environment. 116(1-2): 72-84. Chang, C. C., Yang, M. H., Wen, H. M., and Chern, J. C. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 10(3). Chen, M., Yang, G., Sheng, Y., Li, P., Qiu, H., Zhou, X., Huang, L., and Chao, Z. (2017). Glomus mosseae inoculation improves the root system architecture, photosynthetic efficiency and flavonoids accumulation of liquorice under nutrient stress. Frontiers in Plant Science. 8: 931. Deng, J., Cheng, W., and Yang, G. (2011). A novel antioxidant activity index (AAU) for natural products using the DPPH assay. Food Chemistry. 125(4): 1430-1435. Franken, P. (2012). The plant strengthening root endophyte Piriformospora indica: potential application and the biology behind. Applied Microbiology and Biotechnology. 96(6): 1455-1464. James, B., Rodel, D., Lorettu, U., Reynaldo, E., and Tariq, H. (2008). Effect of vesicular arboscular mycorrhiza Jamshidi, M., Ahmadi, H. R., Rezazadeh, S., Fathi, F., Mazandarani, M., and Khaki, A. (2010). Study on phenolics and antioxidant activity of some selected plant of Mazandaran Province [In Persian]. Journal of Medicinal Plants. 9(34). Jurkiewicz, A., Ryszka, P., Anielska, T., Waligorski, P., Bialonska, D., Goralska, K., Tsimilli-Michael, M., and Turnau, K. (2010). Optimization of culture conditions of Arnica montana L.: effects of mycorrhizal fungi and competing plants. Mycorrhiza. 20(5): 293-306. Khan, A. G. (2005). Role of soil microbes in the rhizospheres of plants growing on trace metal contaminated soils in phytoremediation. Journal of Trace Elements in Medicine and Biology. 18(4): 355-364. Klara, A. (1998). Essential nutrients in mature leaf tissues generalized as deficient, sufficient or excessive for various plant species. Journal of the Environmental and Nutritional Sciences. 24(11): 25-27. Lenin, M., Selvakumar, G., Thamizhiniyan, P., and Rajendiran, R. (2010). Growth and biochemical changes of vegetable seedlings induced by arbuscular mycorrhizal fungus. Journal of Experimental Sciences. 1(4). Liu, L., Li, D., Ma, Y., Shen, H., Zhao, S., and Wang, Y. (2021). Combined application of arbuscular mycorrhizal fungi and exogenous melatonin alleviates drought stress and improves plant growth in tobacco seedlings. Journal of Plant Growth Regulation. 40: 1074-1087. Mahaffee, W. F., and Kloepper, J. W. (1994). Applications of plant growth promoting rhizobacteria in sustainable agriculture. Soil biota: management in sustainable farming systems, Pankburst, C.E., Double, B.M., Gupta, V.V.S.R., and Grace, P.R., eds. CSIRO, Pub. East Melbourne, Australia. 23-31. Mathur, N., and Vyas, A. (1995). Influence of VA mycorrhizae on net photosynthesis and transpiration of Ziziphus mauritiana. Journal of plant physiology. 147(3-4): 328-330. Mohammad, M. J., Malkawi, H. I., and Shibli, R. (2003). Effects of arbuscular mycorrhizal fungi and phosphorus fertilization on growth and nutrient uptake of barley grown on soils with different levels of salts. Journal of Plant Nutrition. 26(1): 125-137. Mota-Fernandez, S., Alvarez-Solis, J. D., Abud-Archila, M., Dendooven, L., and Gutierrez-Miceli, A. F. (2011). Effect of arbuscular mycorrhizal fungi and phosphorus concentration on plant growth and phenols in micro propagated Aloe vera L. plantlets. Journal of Medicinal Plants Research. 5: 6260-6266. Nadjafi, F., Koocheki, A., Honermeier, B. and Asili, J. (2009). Autecology, Ethnomedicinal and phytochemical studies of Nepeta binaloudensis Jamzad a highly endangered medicinal plant of Iran. Journal of Essential Oil Bearing Plant. 12: 37-41. Nell, M., Voetsch, M., Vierheilig, H., Steinkellner, S., Zitterl-Eglseer, K., Franz, C., and Novak, J. (2009). Effect of phosphorus uptake on growth and secondary metabolites of garden sage (Salvia officinalis L.). Journal of the Science of Food and Agriculture. 89(6): 1090-1096. Ozkan, S. B., Wu, G. A., Chodera, J. D., and Dill, K. A. (2007). Protein folding by zipping and assembly. Proceedings of the National Academy of Sciences. 104(29): 11987-11992. Pedonee-Bonfim, M. V. L., da Silva, F. S. B., and Maia, L. C. (2015). Production of secondary metabolites by mycorrhizal plants with medicinal or nutritional potential. Acta Physiologiae Plantarum. 37: 1-12. Saha, H., Kaloterakis, N., Harvey, J. A., Van der Putten, W. H., and Biere, A. (2022). Effects of light quality on colonization of tomato roots by AMF and implications for growth and defense. Plants. 11(7): 861. Sanchez-Bianco, M.J., Ferrandez, T., Morales, M.A., Morte, A. and Alarcon, J.J. (2004). Variations in water status, gas exchange, and growth in Rosmarinus officinalis plants infected with Glomus deserticolaunder drought conditions. Journal of Plant Physiology. 161: 675-682. Sannazzaro, A. I., Ruiz, O. A., Alberto, E. O., and Menéndez, A. B. (2006). Alleviation of salt stress in Lotus glaber by Glomus intraradices. Plant and Soil. 285(1): 279-287. Singleton, V. L., Orthofer, R., and Lamuela-Raventos, R. M. (1999). [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In Meethods in Enzymology. 299: 152-178. Smith, S. E., Facelli, E., Pope, S., and Andrew-Smith, F. (2010). Plant performance in stressful environments: interpreting new and established knowledge of the roles of arbuscular mycorrhizas. Plant and Soil. 326(1): 3-20. Soares, A. C. F., Martins, M. A., Mathias, L., and Freitas, M. S. M. (2005). Arbuscular mycorrhizal fungi and the occurrence of flavonoids in roots of passion fruit seedlings. Scientia Agricola. 62: 331-336. Soobrattee, M. A., Bahorun, T., Neergheen, V. S., Googoolye, K., and Aruoma, O. I. (2008). Assessment of the content of phenolics and antioxidant actions of the Rubiaceae, Ebenaceae, Celastraceae, Erythroxylaceae and Sterculaceae families of Mauritian endemic plants. Toxicology in vitro. 22(1): 45-56. Thakur, A. K., and Panwar, J. D. S. (1997). Response of Rhizobium-vesicular arbuscular mycorrhizal symbionts on photosynthesis, nitrogen metabolism and sucrose translocation in greengram (Phaseolus radiatus). The Indian Journal of Agricultural Sciences. 67(6); 245-248. Toussaint, J. P., St-Arnaud, M., and Charest, C. (2004). Nitrogen transfer and assimilation between the arbuscular mycorrhizal fungus Glomus intraradices Schenck and Smith and Ri T-DNA roots of Daucus carota L. in an in vitro compartmented system. Canadian Journal of Microbiology. 50: 251-260. Tussiant, J. P., Smith, F. A., and Smith, S. E. (2007). Arbuscular mycorrhizal fungi can induce the production of phytochemicals in sweet basil irrespective of phosphorus nutrition. Mycorrhiza. 17: 291-297. Valentine, A. J., Mortimer, P. E., Lintnaar, M., and Borge, R. (2006). Drought responses of arbuscular mycorrhizal grapevines. 41: 127-133. Varma, A. (1999). Functions and application of arbuscular mycorrhizal fungi in arid and semi-arid soils. In Mycorrhizal. Springer, Berlin, Heidelberg. 521-556. Wu, C., Qu, J., Liu, L., Kang, H., Sun, H., Zhang, Y., Ghorbani, A., and Pehlivan, N. (2021). Que vadis: signaling molecules and small secreted proteins from mycorrhizal fungi at the early stage of mycorrhiza formation. Symbiosis.85: 123-143. Xie, K., Ren, Y., Chen, A., Yang, C., Zheng, Q., Chen, J., Wang, D., Li, Y., Hu, S., and Xu, G. (2022). Plant nitrogen nutrition: The roles of arbuscular mycorrhizal fungi. Journal of Plant Physiology. 269: 153591. Younesi, O., and Moradi, A. (2015). The effects of growth promoting bacteria (GPB) and mycorrhizal fungi on seedling emergence, early establishment and growth of alfalfa (Medicago sativa) under salinity stress condition. Journal of Plant Production Research. 22(1): 105-125. | ||
|
آمار تعداد مشاهده مقاله: 185 تعداد دریافت فایل اصل مقاله: 132 |
||
