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بررسی اثر نانوذره اکسید مس بر فعالیت و ساختار آنزیم پراکسیداز لیپیدیوم درابا (LDP) | ||
| زیست شناسی کاربردی | ||
| مقاله 6، دوره 36، شماره 1 - شماره پیاپی 75، خرداد 1402، صفحه 135-158 اصل مقاله (1.45 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2023.41224.1500 | ||
| نویسندگان | ||
| علی ریاحی مدوار* 1؛ فرشید برزگری دهج2؛ رضا میرزایی2؛ فهیمه بهرام نژاد3 | ||
| 1دانشیار ،گروه زیست شناسی سلولی-مولکولی، دانشکده علوم پایه، دانشگاه کوثر بجنورد، بجنورد، | ||
| 2دانش‌آموخته کارشناسی ارشد، گروه بیوتکنولوژی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران. | ||
| 3استادیار، دانشگاه علوم پزشکی جیرفت، جیرفت، ایران. | ||
| چکیده | ||
| مقدمه: ورود نانوذرات به بدن موجودات زنده می‌تواند بر روی ماکرومولکول‌های زیستی اثر گذاشته و حیات آنها را تحت تاثیر قرار دهد. در این مطالعه، تغییرات فعالیت و ساختار آنزیم نوترکیبLDP در حضور نانوذره اکسید‌مس (nCuO) مورد آنالیز قرار گرفت. روش‌ها: فعالیت آنزیم در حضور غلظت‌های مختلف nCuO اندازه‌گیری شد. از مطالعات فلورسانس ذاتی در حضور غلظت‌های مختلف نانوذره و دماهای مختلف جهت بررسی تغییرات ساختاری و تعیین پارامترهای ترمودینامیکی کمپلکس آنزیم-نانوذره استفاده شد. نتایج و بحث: نتایج نشان داد که فعالیت آنزیم در حضور nCuO افزایش می‌یابد. شدت فلورسانس ذاتی در حضور نانوذره در روندی وابسته به غلظت کاهش یافت. آنالیزهای ترمودینامیکی نشان دهنده مثبت بودن آنتالپی و آنتروپی و منفی بودن تغییرات انرژی آزاد واکنش می‌باشند. بر اساس نتایج، تغییر فعالیت آنزیم، حاصل تغییرات ساختاری آنزیم در میانکنش با نانوذره است که این فرایند با استفاده از میانکنش‌های هیدروفوب و بصورت خودبخودی پیش می‌رود. | ||
| کلیدواژه‌ها | ||
| پارامترهای ترمودینامیکی؛ فلورسانس؛ میانکنش‌های هیدروفوب | ||
| عنوان مقاله [English] | ||
| Investigation of the CuO nanoparticles effect on activity and structure of Lepidium draba peroxidase enzyme | ||
| نویسندگان [English] | ||
| Ali Riahi-Madvar1؛ Farshid Barzegary-Dehaj2؛ , Reza Mirzaee2؛ fahiemeh bahramnejad3 | ||
| 1Associate Professor,Department of Molecular and Cell Biology, Faculty of Basic Sciences, Kosar University of Bojnord, Bojnord, Iran | ||
| 2Master's student, Department of Biotechnology, Research Institute of Advanced Science and Technology and Environmental Sciences, Post Graduate University of Industrial and Advanced Technology, Kerman, Iran. | ||
| 3Assistant Professor, Jiroft University of Medical Sciences, Jiroft, Iran. | ||
| چکیده [English] | ||
| Introduction: Entrance nanoparticles into the environment provides possibility of their entry into living organisms body. Their entry into the body can affect biological macromolecules and affect their life. Here, to investigate effect of copper oxide nanoparticle (nCuO) on recombinant Lepidium draba peroxidase, changes in enzyme activity and structure were analyzed in the presence of nCuO. Methods: Enzyme activity was measured in the presence of different concentrations of nCuO and repeated three times. Results were statistically analyzed using SPSS software at a significance level of 5%. Intrinsic fluorescence studies in the presence of nanoparticles were utilized to investigate structural changes of the enzyme. These studies were done at different temperatures to determine thermodynamic parameters such as the type of forces involved in the nanoparticle-enzyme interaction. Results and discussion: Enzyme activity in the presence of nCuO significantly increased at the level of 5%. The most enzyme activity were attributed to the concentration ranges of 80-180 nM nanoparticles, which it increased more than 46% compared to the control. Intrinsic fluorescence intensity decreased in the manner nanoparticles concentrations increased in media. Decrease intrinsic fluorescence emission indicates changes in the enzyme structure in presence of nanoparticle. Thermodynamic parameters including entropy (∆S°) and enthalpy (∆H°) related to the interaction of enzyme with nanoparticle were calculated as 0.108 and 3.81 respectively. The positive sign of these parameters indicates the important role of hydrophobic interactions in this process. On the other hand, negativity of free energy (∆G°) changes indicates that this process is exergonic and proceeds spontaneously. | ||
| کلیدواژه‌ها [English] | ||
| Fluorescence, Hydrophobic interactions, Thermodynamic parameters | ||
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