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ترسیب کربن با کربنیک آنهیدراز نوترکیب در باکتری Ralstonia eutropha | ||
| زیست شناسی کاربردی | ||
| مقاله 9، دوره 33، شماره 2 - شماره پیاپی 64، شهریور 1399، صفحه 123-138 اصل مقاله (866 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2019.25636.1295 | ||
| نویسندگان | ||
| محدثه محسن پور1؛ نور امیرمظفری* 2؛ زهرا نورمحمدی3؛ شیوا ایرانی4 | ||
| 1دانشجوی دکتری،گروه زیست شناسی واحد علوم و تحقیقات ، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 2استاد ،دانشگاه علوم پزشکی ایران، دانشکده پزشکی، گروه میکروب‌شناسی، تهران، ایران | ||
| 3دانشیار ، گروه زیست شناسی واحد علوم و تحقیقات ، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 4دانشیار ،گروه زیست شناسی واحد علوم و تحقیقات ، دانشگاه آزاد اسلامی، تهران، ایران | ||
| چکیده | ||
| انتشار گازهای گلخانه‌‌ای و پلاستیک‌ها از مهمترین معضلات زیست محیطی هستند. هدف، بررسی باکتری Ralstonia eutrophaبه عنوان میزبان آنزیم کربنیک‌آنهیدراز برای تولید پلی‌هیدروکسی‌بوتیرات (PHB) است. توالی ژن آنزیم کربنیک‌آنهیدراز (CA) در وکتور بیانی کلون‌سازی و به R. eutropha منتقل شد. تولید PHB در باکتری‌ها با روش‌های FT-IR و GC-MS بررسی شد.میزان PHB در باکتری نوترکیب درحضور CO2نسبت‌به باکتری نوترکیب در غیاب CO2، 49/31 درصد و نسبت‌به باکتری غیرنوترکیب درحضور و عدم حضور CO2 به‌ترتیب 44/18 و 44/34 درصد افزایش داشت. تولید PHB در باکتری نوترکیب در محیط محدود درحضور CO2 نسبت‌به باکتری نوترکیب در غیاب CO2، 57/26 درصد و نسبت‌به باکتری غیرنوترکیب درحضور و عدم حضور CO2به‌ترتیب 30/18 و 63/30 درصد افزایش داشت.نتایج سنجش PHB نشان‌دهنده مؤثر بودن بیان CA در افزایش PHB در R. eutropha بود. همچنین باکتری حاصل می‌تواند درحضور CO2 در یک محیط ساده، علاوه بر ترسیب‌کربن،PHB تولید کند. | ||
| کلیدواژه‌ها | ||
| ترسیب کربن؛ کربنیک آنهیدراز؛ Ralstonia eutropha | ||
| عنوان مقاله [English] | ||
| Carbon sequestration by recombinant carbonic anhydrase in Ralstonia eutropha | ||
| نویسندگان [English] | ||
| Mohaddeseh Mohsenpour1؛ Nour Amirmozafari2؛ Zahra Noormohammadi3؛ Shiva Irani4 | ||
| 1PhD student.Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Professor,Department of Microbiology, Faculty of Medicine, Iran University of Medical Science and Health Services, Tehran, Iran | ||
| 3Associate professor.Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 4Associate professor.Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده [English] | ||
| Emission of greenhouse gases and plastic wastes are one of the most important environmental problems in the world. The purpose of this study was to use the bacterium Ralstonia eutropha as the host for recombinant carbonic anhydrase enzyme which is used for carbon sequestration and poly hydroxyl butyrate (PHB) production. The carbonic anhydrase gene (CA) was inserted in the expression vector and transformed into Ralstonia eutropha. Then PHB production was assayed by FT-IR and GC-MS methods in recombinant and non-recombinant bacteria in different concentrations of LB medium in the presence and absence of CO2. Production of PHB in LB medium increased 31.49 percent in recombinant bacteria in the presence of CO2. These increases were 18.44 and 34.44 percent compared to non-recombinant bacteria in presence and absence of CO2, respectively. In 0.5 LB medium, the recombinant bacterium produced 26.57 percent PHB in presence of CO2 more than in its absence. Non-recombinant bacterium in this medium produced 18.30 and 30.63 percent PHB less than the recombinant cell in the presence and absence of CO2, respectively. The quantity of PHB production using GC-MS revealed that CA gene expression was effective for increasing PHB production in recombinant R. eutropha. In the presence of CO2 and by using a simple medium. The recombinant bacterium is able to produce PHB. This bacterium can also be effective in carbon sequestration. | ||
| کلیدواژه‌ها [English] | ||
| Carbonic anhydrase, Carbon sequestration, Ralstonia eutropha | ||
| مراجع | ||
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Aneja, K.K., Ashby, R.D. and Solaiman, D.K. (2009). Altered composition of Ralstonia eutropha poly(hydroxyalkanoate) through expression of PHA synthase from Allochromatium vinosum ATCC 35206. Biotechnology Letters, 31: 1601–1612. Bi, C., Su, P. and Muuller, J. (2013). Development of a broad host synthetic biology toolbox for Ralstonia eutropha and its application to engineering hydrocarbon biofuel production. Microbial Cell Factories, 12: 107-116. Borchert, M. and Saunders, P. (2013). Heat-stable carbonic anhydrases and their use. Patent US 8697428 B2. Bose, H. and Satyanarayana, T. (2017). Microbial carbonic anhydrases in biomimetic carbon sequestration for mitigating global warming: prospects and perspectives, Frontiers in Microbiology, 8: 1615-1635. Chamani Mohasses, F., Solouki, M., Ghareyazie, B., Fahmideh, L., Mohsenpour, M. (2020). Correlation between gene expression levels under drought stress and synonymous codon usage in rice plant by in-silico study. PLoS ONE, 15: 1-21. Crepin, L., Lombard, E. and Guillouet, S.E. (2016). Metabolic engineering of Cupriavidus necator for heterotrophic and autotrophic alka(e)ne production. Metabolic Engineering, 37: 92–101. Ewering, C., Heuser, F. and Benuolken, J.K. (2006). Metabolic engineering of strains of Ralstonia eutropha and Pseudomonas putida for biotechnological production of 2-methylcitric acid. Metabolic Engineering, 8: 587–602. Hassan, M.A., Bakhiet, E.K., Ali, S.G. and Hussien, H.R. (2016). Production and characterization of polyhydroxybutyrate (PHB) produced by Bacillus sp. isolated from Egypt. Journal of Applied Pharmaceutical Science, 6: 046-051. Jo, B. H. (2016). Engineering de novo disulfide bond in bacterial α-type carbonic anhydrase for thermostable carbon sequestration. Scientific Reports, 6: 29322-29330. Juengert, J.R., Bresan, S. and Jendrossek, D. (2018). Determination of polyhydroxybutyrate (PHB) content in Ralstonia eutropha using gas chromatography and nile red Staining. BioProtocol, 8: 2748-2762. Kumar, M., Gupta, A. and Thakur, I.S. (2016). Carbon dioxide sequestration by chemolithotrophic oleaginous bacteria for production and optimization of polyhydroxyalkanoate. Bioresource Technology. 213: 249–256. Kumar, M., Sundaram, S., Gnansounou, E., Larroche, C. and Thakur, I.S. (2018). Carbon dioxide capture, storage and production of biofuel and biomaterials by bacteria: A review, Bioresource Technology, 247: 1059-1068. Kumar, V., and Satyanarayana, T. (2014). Production of thermo-alkali-stable xylanase by a novel polyextremophilic Bacillus halodurans TSEV1 in cane molasses medium and its applicability in making whole wheat bread. Bioprocess. Biosystems Engineering, 37: 1043–1053. Lu, J., Brigham, C.J. and Gai, C.S. (2012). Studies on the production of branched-chain alcohols in engineered Ralstonia eutropha H16. Applied Microbiology and Biotechnology, 96: 283–297. Mesbah, N. M., and Wiegel, J. (2012). Life under multiple extreme conditions: diversity and physiology of the halophilic alkalithermophiles. Appl. Environmental Microbiology, 78: 4074–4082. Mitchell, A. C., Dideriksen, K., Spangler, L. H., Cunningham, A. B. and Gerlach, R. (2010). Microbially enhanced carbon capture and storage by mineral-trapping and solubility-trapping. Environmental Science and Technology, 44: 5270–5276. Mohsenpour, M., Babaeian, J.N., Touhidfar, M. and Habashi A.A. (2008). Design and construction of four recombinant plasmid vectors containing chitinase, glucanase and BT genes, suitable for plant transformation. Journal of Agricultural Sciences and Natural Resources, 15: 69-80 Mohsenpour, M., Noormohammadi, Z., Irani, S. and Amirmozafari, N. (2019). Expression of an environmentally friendly enzyme, engineered carbonic anhydrase, in Escherichia coli. International Journal of Environmental Research, 3: 295-301. Mohsenpour, M., Tohidfar, M., Jelodar, N.B. and Jouzani G.S. (2015). Designing a new marker-free and tissue-specific platform for molecular farming applications. Journal of Plant Biochemistry and Biotechnology, 24: 433-440. Muuller, J., MacEachran, D. and Burd, H. (2013). Engineering of Ralstonia eutropha H16 for autotrophic and heterotrophic production of methyl ketones. Applied and Environmental Microbiology, 79: 4433–4439. Overhage, J., Steinbuuchel, A. and Priefert, H. (2002). Biotransformation of eugenol to ferulic acid by a recombinant strain of Ralstonia eutropha H16. Applied and Environmental Microbiology, 68: 4315–4321. Raberg, M., Volodina, E., Lin, K. and Steinbuchel, A. (2017). Ralstonia eutropha H16 in progress: Applications beside PHAs and establishment as production platform by advanced genetic tools. Critical Reviews in Biotechnology, 38: 494-510. Rohwerder, T and Muuller. R.H. (2010). Biosynthesis of 2-hydroxyisobutyric acid (2-HIBA) from renewable carbon. Microbial Cell Factories, 9: 13-22. Sambrook, J. & Rusell, D. (2001). Molecular cloning: a laboratory manual (3th Ed.). New York: Cold Spring Harbor Laboratory. Schirmer, A., Rude, M.A. and Li, X. (2010). Microbial biosynthesis of alkanes. Science, 329: 559–562. Voss, I. and Steinbuuchel, A. (2006). Application of a KDPG-aldolase gene-dependent addiction system for enhanced production of cyanophycin in Ralstonia eutropha strain H16. Metabolic Engineering, 8: 66–78. Tohidfar, M., Ghoreyshi, E., Fakheri, B. and Mohsenpour, M. (2014). Design and construction of specific chloroplast vectors for rice plants containing betaine aldehyde dehydrogenase (badh) and flavodoxin (fld) genes for resistance to abiotic stress. Crop Biotechnology, 4: 47-59. Zhang, Z., Lian, B., Hou,W., Chen,M., Li, X. and Shen,W. (2011). Optimization of nutritional constituents for carbonic anhydrase production by Bacillus mucilaginosus K02. African Journal of Biotechnology. 10: 8403–8413.
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