پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 25 |
| تعداد شماره‌ها | 967 |
| تعداد مقالات | 8,010 |
| تعداد مشاهده مقاله | 13,573,814 |
| تعداد دریافت فایل اصل مقاله | 9,616,690 |
بررسی اثر نیترات نقره بر ترکیبات و فعالیت آنتی اکسیدانی در کالوسهای حاصل از جدا کشت برگ گیاه اسطوخودوس(Lavandula angostifolia) | ||
| زیست شناسی کاربردی | ||
| مقاله 8، دوره 30، شماره 1، شهریور 1396، صفحه 137-158 اصل مقاله (508.59 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2017.7213.1009 | ||
| نویسندگان | ||
| فیروزه علیرضایی* 1؛ خدیجه کیارستمی2؛ منیر حسین زاده نمین3 | ||
| 1فارق ااتحصیل از مقطع کارشناسی ارشد رشته فیزیوژی گیاهی،دانشکده علوم زیسی،دانشگاه الزهرا، ایران | ||
| 2مدیر گروه فیزیولوژی گیاهی،دانشکده علوم زیستی ،دانشگاه الزهرا،ایران | ||
| 3استادیار گروه فیزیولوژی گیاهی، دانشکده علوم زیستی، دانشگاه الزهرا، ایران | ||
| چکیده | ||
| اسطوخودوس با نام علمی Lavandula angustifolia گیاهی است متعلق به خانواده نعنا عیان ، تمام اندام های این گیاه غنی از ترکیبات ثانویه با خاصیت آنتی اکسیدانی بالا است و می تواند منبع مناسبی برای تهیه آنتی اکسیدانها در شرایط کشت بافت باشد. در این بررسی با هدف افزایش متابولیت های ثانویه با خاصیت آنتی اکسیدانی از الیسیتور غیر زیستی نیترات نقره استفاده شد. میزان 1،2،4و6mg/l نیترات نقره به کالوسهای یک ماهه رشد کرده در محیطMS دارای هورمون های نفتالن استیک اسید (NAA ) mg/l 2وبنزیل آمینوپورین(BAP)mg/l 4 اضافه شد و پس از یک ماه کالوسها برداشت و آنالیزها انجام شد. نتایج نشان داد با افزایش غلظت نیترات نقره در محیط کشت،میزان رشد کالوسها افزایش یافت و افزایش میزان نیترات نقره تا غلظت mg/l 4سبب افزایش میزان رزمارینیک اسید ،ترکیبات فنلی و فلاونوئید و همچنین فعالیت آنتی اکسیدانی شد،اما در غلظت های بالاتر از mg/l4 اثرات سمی یونهای نقره آشکار شد و باعث کاهش میزان رزمارینیک اسید ،ترکیبات فنلی و همچنین فعالیت آنتی اکسیدانی شد. | ||
| کلیدواژه‌ها | ||
| اســطوخودوس؛ رزمارینیــک اســید؛ ترکیبــات فنلــی؛ فلاونوئیــد؛ فعالیــت آنتــی اکســیدانی | ||
| عنوان مقاله [English] | ||
| Evaluation effect of silver nitrate on antioxidant activity and antioxidant compounds in calli frome leaves of lavandula angostifolia. | ||
| نویسندگان [English] | ||
| Firuze Alirezayi1؛ Khadijah Kiarostami2؛ Monia Hussein Zadeh Namin3 | ||
| 1Graduate from the Master's degree in Plant Physiology, Faculty of Science, Zazi University, Alzahra University, Iran | ||
| 2Director of the Department of Plant Physiology, Faculty of Life Sciences, Al-Zahra University, Iran | ||
| 3Assistant Professor of Plant Physiology, Faculty of Life Sciences, Alzahra University, Iran | ||
| چکیده [English] | ||
| Lavender with scientific name of Lavandula angustifolia belongs to Lamiaceae family ،all parts of this plant are rich of secondary compounds with high antioxidant properties and can be a good source for providing antioxidants in tissue culture conditions.In this research with the aim of increasing plants secondry methabolites with high antioxidant properties ,silver nitrate as abiotic elicitor used in tissue culture of Lavandula angutifolia. Concentrations of 1, 2, 4 and 6 mg/l of silver nitrate as abiotic elicitor added to the one-month calli from leaf explant grown in MS medium with , Naphthaleneacetic acid(NAA)2mg/l, Benzyl amino purine (BAP) 4mg/l.After a month calli were harvested and the analyses were performed.The results showed that with increasing concentration of silver nitrate in the culture medium, growth of calli was increased and increasing the amount of silver nitrate concentration up to 4mg / l, increased Rosmarinic acid, flavonoids ,phenolic compounds and antioxidant activity .But in higher concentration of 4 mg / l the toxic effects of silver ions revealed and reduced the amount of Rosmarinic acid,phenolic compounds and antioxidant activity. | ||
| کلیدواژه‌ها [English] | ||
| Lavendula angostifolia, Rosmarinic acid, phenolic compounds, flavonoids, anti� oxidant activity | ||
| مراجع | ||
|
اصغری،غ.،مستاجران،ا.،نخـعی،ا.(1390).تأثیر نیـترات نقره و سالیسیلیک اسید بر تولید تاکسول در گیاه .Taxus baccata L..فصلنامه گیاهان دارویی شماره هشت،74-82 شریفی،م.،اسماعیل زاده بهبهانی،ص.(1392). افزایش تولید متابولیتهای ثانوی گیاهی با استفاده از الیسیتورهای زیستی. مجله سلول و بافت ،119-128. Begum, A.A. Tamaki, M. Kako, S(1994) " Formation of protocorm-like bodies (PLBs) and shoot development through in vitro culture of outer tissue of Cymbidium PLB” Journal of the Japanese Society for Horticultural Science 63(3):663–673. Beyer,E.M. (1976)”Silver ion:a potent anti-ethylene agent in cucumber and tomato” Horticulture Science 11(3):175-196. Boudet,A.M.(2007)”Evolution and current status of research in phenolic compounds” Phytochemistry 68: 2722-2735. Cheon Chae ,S and Park, S. (2012)” Improved shoot organogenesis of Echinacea angustifolia DC treated with ethylene inhibitors” Life Science Journal 9(4):1725-1728. Creasy,L.L.(1976)” Phenylalanine ammonium lyase-inactivating system in sunflower system in sunflower leaves” Phytochemistry15: 673–675. Conde,E.,Cadahfa, E and .GarciaVallego ,M.C. (1995) ”HPLC analysis of Flavonoids and phenolic acid and aldehydes in Eucalyptus spp” Chromatogaraphia 41: 657.660. Cuvelier, M. E., Richard, H and Berset ,C. (1996)” Antioxidative activity and phenolic composition of pilot-plant and commercial extracts of sage and rosemary” Journal of American oil Chemist Society 73:645-652. Diplock, A. T., Charleux ,G.L.,, Crozier.Willi, G., Kok, F.J., Rice-Evans,C., Roberfroid,M and Vina -Ribes, J. (1998)”Functional food science and defence against reactive oxidative species” British Journal of Nutrition 80:77-112. Elumalai ,EK., Prasad ,TN., Kambala ,V.,Nagajyothi, PC and David, E. (2010)” Green synthesis of silver nanoparticle using Euphorbia hirta L. and their antifungal activities” Applied Science Research 2(6): 76-81. Elzaawely , A and Tawata, S. (2007)” Changes in essential oil, kava pyrones and total phenolics of Alpinia zerumbet (Pers.) B.L. Burtt. & R.M. Sm. Leaves exposed to copper sulphate” Environmental and Experimental Botany 59: 347-353. Furden, B., Humburg, A and Fuss, E. (2005)”Influence of methyl jasmonate on podophyllotoxin and 6-methoxypodophyllotoxin accumulation in Linum album cell suspension cultures” Plant Cell Reports 24: 312-317. Ghanati , F ., Bakhtiarian , S., Mohammadparast ,B and Keyhani, M. (2014)”Production of New Active Phytocompounds by (Achillea millefolium L.) after Elicitation with Silver Nanoparticles and Methyl Jasmonate” Journal of Bioscience Biotechnology Research Asia 11(2):391-399. Gokhale, M and Bansal Y.K; S.(2010)” Assessment of Secondary Metabolites in In vitro Regenerated Plantlets of Oroxylum indicum L. Vent” Plant Tissue Culture & Biotechnology. 20(1): 21-28. Hausler, E.,Petersen , M and Alfermann ,A. (1993)”Isolation of protoplasts and vacuoles from suspension cultures of (Coleus blumei Benth)” Plant Cell Reports12: 51-59. Jiao, Z.,Liu ,J and Wang ,S. (2005)” Antioxidant activities of total pigment extract from Blackberries” Food Technology and Biotechnology 43: 97-102 Jiravova .J., Tomankova, K.B., Harvanova, M., Malina, L., Malohlava ,4., Luhova ,L., Panacek ,A., Manisova, B and Kolarova H.(2016). The effect of silver nanoparticles and silver ions on mammalian and plant cells in vitro. Food and chemical toxicology 96:50-61. Kathleen ,B ;(2012)”Elucidating the Effect of Silver on Ethylene Signaling in Arabidopsis thaliana” Masters Theses. University of Tennessee, Knoxville. Kaveh R., Li ,YS., Ranjbar ,S., Tehrani .R., Brueck ,C.L and Van Aken, B.(2013)”Changes in Arabidopsis thaliana Gene Expression in Response to Silver Nanoparticles and Silver Ions” Environmental Science & Technology 47(18): 10637-10644 Kulisic ,T.,Dragovic-Uzelac ,V.,Milos ,M.(2006)” Antioxidant activity of aqueous tea infusions prepared from oregano, thyme and wild thyme” Food Technology Biotechnology 44: 485-492. Kushad, M.M.,Poovaiah, B.W.(1984)”Deferal of senescence and abscission by chemical inhibition of ehtylene synthesis and action in bean explants” Plant Physiology 76(2):293-296. Lakshmi, V., Devlina ,D., Nilanjana ,D and Vimala ,R. (2013)” Studies on Toxicity of Ag (I) on Plants and Microbes “Research Journal of Pharmaceutical, Biological and Chemical Sciences 4(4):176-188. Lee ,S,Y., Xu, H., Kim, Y.K and Park, SU; (2008)” Rosmarinic acid production in hairy root cultures of (Agastache rugose) Kuntze” World Journal of Microbiology and Biotechnology24: 969-972. Liao, X., Tang ,L and Zhang, F. (2012)”Effect of silver nitrate on callus regeneration and activity of some enzymes during tissue culture of wheat immature inflorescence” Journal of sciences paper online 1-14 Lopez-Arnaldos,T., Lopez-Serrano, M., Ros.,Barcelo,A.,Calderon,A. A. and Zapata ,J. M. (1995)"Spectropho-ometric determination of rosmarinic acid in plant cell cultures by complexation with Fe2+ ions" Fresenius' Journal of Analytical Chemistry 351: 311-314.. López Arnaldos ,T., Muñoz ,R., Ferrer ,M.A and Calderón, A.A. (2001)”Change in phenol content during Straberry callus” plant physiology113:315-322 Misawa ,N., Truesdale, M.R., Sandmann, G., Fraser, P.D.,Bird ,C., Schuch ,W and Bramley ,P.M. (1994)” Expression of a tomato cDNA coding for phytoene synthase in Escherichia coli, phytoen formation in vivo and in vitro and functional analysis of the various truncated gene products” The Journal of Biochemistry 116:980-985 Molyneux, P. (2004)”The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity” Songklanakrin Journal of Science and Technology 26: 211–219. Moses ,T and Mukundan, U. (2013)”Elicitation of Thymol in Thymus Vulgaris, A Medicinally Important Plant” International Journal of Drug Discovery and Herbal Research 3:590-595. Moure , A., Cruz , J.M., Franco , D., Domínguez , J.M., Sineiro , J., Domínguez , H., José, Núñez , M and Parajó , J.C. (2001)”Natural antioxidants from residual sources” Food Chemistry 72(2): 145-171. Murashige, T and Skoog, F. (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures". Physiologia Plantarum. 15 (3): 473–497. Nejatzadeh-Barandozi, F., Darvishzadeh, F and Aminkhani, A. (2014)”Effect of nano silver and silver nitrate on seed yield of (Ocimum basilicum L.)” Organic and Medicinal Chemistry Letters 4:11( open Acess( Niciforovic, N., Mihailovic, V., Maskovic, P.. Solujic,S., Stojkovic, A and Pavlovic-Muratspahic, D.(2010) ”Antioxidant activity of selected plant species; potential new sources of natural antioxidants” Food and Chemical Toxicology 48:3125-30.
Nobre , J. (1996) ”In vitro cloning ad micropropagationof Lavandula stoechas from field grown plants” Plant Cell Tissue and Organ Culture, 46:151- 155. Park, S ., Uddin, M., KimYong, K and Lee ,S. (2008)”Biotechnological applications for rosmarinic acid production in plant ” African Journal of Biotechnology 7 (25):4959-4965 Pereira, D.M.,Valentão , P.,Pereira, J.A., Andrade, P.B. (2009)” Phenolics compounds From chemistry to biology” Molecules 14(6): 2202-2211. Perron, N and Brumaghim,J. A. (2009)” Review of the antioxidant mechanisms of polyphenol compounds related to iron binding” Cell Biochemistry and Biophysics 53:75-100. Petersen ,M. (1994)” Coleus spp. In vitro culture and the production of forskolin and rosmarinic acid” Medical and Aromatic Plants 26: 69-93. Radhakrishnan, T. O., Jithesh, A and Dobaria ,J. R.(2014)”Hight Fequency regeneration protocol for callus culture of peanut leaves using ethylene modulator as culture medium” Aaditive” The Bioscan 9(2): 599-604. Ramachandra,CT and SrinivasaRao, P.(2008)”Processing of Aloe vera leaf gel” American Journal of Agricultural and Biological Sciences 3(2):502-510. Ravishankar, G.A and Rao, S.R. (2002)”Plant cell cultures: chemical factories of secondary metabolites” Biotechnology Advances 20 (2):101-153. Rice-Evans ,C. A., Miller ,J. M and Paganga ,G. (1996) ”Structure-antioxidant activity relationship of flavonoids and phenolic acids” Free Radical Biology and Medicine 20:933-956. Soobrattee, M., Neergheen ,V ., Luximon-Ramma , A., Aruoma , O and Bahorun , T.(2005)” Phenolics as potential antioxidant therapeutic agents Mechanism and actions” Mutation Research 579: 200-213. Steephen ,M., Nagarajan ,S and Ganesh ,D. (2010)” Phloroglucinol and silver nitrate enhances axillary shoot proliferation in nodal explants of Vitex negundo L. –an aromatic medicinal plant” Iranian Journal of Biotechnology 8(2):82-89. Szabo, E., Thelen, A and Petersen, M.(1999)”Fungal elicitor preparations and methyljasmonate enhance rosmarinic acid accumulation in suspension cultures of Coleus blumei Plant Cell Reports 18 : 485–489 Tognoni , F and Panizza, M.(1988) ”Clonal propagation, callus Formation and plant Regeneration of Lavandin” Scientia Horticulturae 37: 157 163. Turhan, H .(2004)” Effect of silver nitrate (ethylene inhibitor) in in vitro tissue culture of potato (Solanum tubersum)” Bioechnology 20(4);72-74. Vogt,T.(2010)” Phenylpropanoid biosynthesis in plants” Molecular Plant” 3(1):2-20. Wang ,K.L., Hai , C.L and Ecker , J.R.(2002)” Ethylene biosynthesis and signaling networks”The Plant Cell 14:131-151. Yan, Q., Shi ,M., Neg ,J and Wu ,J.(2006)”Elicitor-induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Salvia miltiorrhiza hairy roots” Plant Science 170: 853–858. | ||
|
آمار تعداد مشاهده مقاله: 1,086 تعداد دریافت فایل اصل مقاله: 1,820 |
||