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سنتز سبز و تعیین ویژگی نانوذرات سولفید نقره توسط باکتریGMS10 Bacillus safensis جدا شده از خاک آلوده معدن طلا | ||
| زیست شناسی کاربردی | ||
| دوره 35، شماره 4 - شماره پیاپی 74، اسفند 1401، صفحه 7-23 اصل مقاله (940.84 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2022.39607.1472 | ||
| نویسندگان | ||
| مراحم آشنگرف* 1؛ سینا داج2 | ||
| 1دانشیار میکروبیولوژی صنعتی گروه علوم زیستی، دانشکده علوم پایه، دانشگاه کردستان، کردستان، سنندج، ایران | ||
| 2دانشجوی کارشناسی ارشد ، میکروبیولوژی صنعتی گروه علوم زیستی، دانشکده علوم پایه، دانشگاه کردستان، کردستان، سنندج، ایران | ||
| چکیده | ||
| مقدمه: این مطالعه با هدف جداسازی و شناسایی باکتری های مقاوم به نقره و بررسی پتانسیل آنها در سنتز نانوسولفید نقره انجام شد. روشها: بررسی اولیه بیوسنتز نانوذرات سولفید نقره از طریق مشاهدات چشمی و بررسی طیف‌های جذبی اسپکتروفتومتری مرئی ماورای بنفش انجام شد. آنالیز میکروسکوپ الکترونی روبشی مجهز به پرتوایکس پاشندۀ با هدف بررسی اندازه، شکل نانوذره و آنالیز عنصری انجام شد. به منظور تعیین گروههای عاملی دخیل در احیای زیستی سولفات نقره به نانوسولفید نقره از طیف سنجی تبدیل فوریه مادون قرمز استفاده شد. نتایج و بحث: براساس نتایج، سویه‌ی باکتریGMS10 Bacillus safensis با بالاترین مقاومت نسبت به سولفات نقره (5 میلی مولار)، قادر به سنتز نانوذرات سولفید نقره کروی شکل با متوسط اندازه‌ی 2/22 نانومتر در غلظت 1 میلی مولار سولفات نقره تحت شرایط بهینه 15 گرم در لیتر زیست توده و 36 ساعت گرماگذاری بود. این مطالعه نخستین گزارش از سنتز سبز نانوذرات سولفید نقره در باکتری B. safensis است. | ||
| کلیدواژه‌ها | ||
| باکتری B. safensis؛ بیوسنتز؛ خاک؛ مشخصه یابی؛ نانوسولفید نقره | ||
| عنوان مقاله [English] | ||
| Green synthesis and characterization of silver sulfide nanoparticles using Bacillus safensis strain GMS10 isolated from contaminated soil of gold mine | ||
| نویسندگان [English] | ||
| Morahem Ashengroph1؛ Sina Daj2 | ||
| 1Associate Professor of Industrial Microbiology, Department of Biological Sciences, Faculty of Basic Sciences, University of Kurdistan, Kurdistan, Sanandaj, Iran | ||
| 2Master's student, Industrial Microbiology, Department of Biological Sciences, Faculty of Basic Sciences, University of Kurdistan, Kurdistan, Sanandaj, Iran | ||
| چکیده [English] | ||
| Introduction: This study aimed to isolate and identify the silver-resistant bacteria and investigation on their potential in the biological synthesis of silver sulfide nanoparticles (Ag2SNPs). Methods: Preliminary characterization of the Ag2SNPs was carried out using visual observations and UV–Visible spectroscopy. Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDX) was used to determine size, morphology, and elemental analysis of the nanoparticles. Fourier-transform infrared spectroscopy (FTIR) analysis was performed to determine the functional groups that are involved in the bioreduction of silver sulfate into Ag2SNPs. Results and discussion: Based on the results, Bacillus safensis strain GMS10 with highest tolerance to silver sulfate (50 mM) was able to synthesize spherical shape of Ag2SNPs with an average size diameter of 22.2 nm under optimized conditions (1 mM silver sulfate, 15 g/L biomass) after 36 hours incubation. This study is the first report on the synthesis of Ag2SNPs using B. safensis. | ||
| کلیدواژه‌ها [English] | ||
| B. safensis, Biosynthesis, Characterization, Silver sulfide nanoparticle, Soil | ||
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