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بررسی اثرات پروتئین هیدرولیز شده کنجاله کانولا بر رشد، ترکیب بدن و بیان ژنهای رشد و اشتهای فیل ماهی (Huso huso) | ||
| زیست شناسی کاربردی | ||
| دوره 35، شماره 1 - شماره پیاپی 71، خرداد 1401، صفحه 20-35 اصل مقاله (697.48 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2021.34652.1404 | ||
| نویسندگان | ||
| محمودرضا ابراهیم نژادعربی1؛ سید مهدی حسینی فرد* 2؛ رضا چنگیزی3؛ صابر وطن دوست4؛ شایان قبادی5 | ||
| 1دانشجوی دکتری شیلات، گروه شیلات، واحد بابل، دانشگاه آزاد اسلامی، بابل، ایران. | ||
| 2استادیار گروه دامپزشکی، واحد بابل، دانشگاه آزاد اسلامی، بابل، ایران. | ||
| 3استادیار گروه شیلات ، دانشکده دامپزشکی دانشگاه آزاد اسلامی واحد بابل | ||
| 4استادیار گروه شیلات، واحد بابل، دانشگاه آزاد اسلامی، بابل، ایران | ||
| 5استادیار گروه شیلات دانشگاه آزاد اسلامی واحد بابل | ||
| چکیده | ||
| این تحقیق با هدف بررسی اثرات افزایش پروتئین هیدرولیز شده کنجاله کانولا بر فاکتورهای رشد، بیان ژنهای GH، IGF و گرلین و ترکیب بدن فیل ماهی میباشد. به همین منظور تعداد 840 قطعه بچه فیل ماهی با میانگین وزنی 5±30 گرم در 12 تانک 2400 لیتری با تعداد 70 عدد در هر تانک توزیع شدند و با سطوح مختلف 0 (شاهد)، 300 (تیمار 1)، 400 (تیمار 2) و 500 (تیمار 3) میلیگرم پروتئین هیدرولیز شده کنجاله کانولا به مدت 8 هفته تغذیه شدند. در پایان دوره پرورش زیستسنجی بهمنظور ارزیابی فاکتورهای رشد و نمونهبرداری از بافت مغز، معده و کبد برای ارزیابی بیان ژنهای مذکور انجام شد. نتایج نشان داد که با افزایش میزان مصرف پروتئین هیذرولیز شده کنجاله کانولا در جیره میزان افزایش وزن بدن، نرخ رشد ویژه و کارایی پروتئین افزایش یافت و بالاترین مقدار در تیمار 3 (500 میلیگرم) بود که اختلاف معنیداری با سایر گروهها داشت (P<0.05) همچنین میزان بیان هر 3 ژن نیز روندی افزایشی را نشان داد که اختلاف بین تیمارها با شاهد معنیدار بود (P<0.05). و بیشترین میزان بیان برای هر سه ژن در تیمار 500 میلیگرم مشاهده شد. در ارتباط با ترکیب شیمیایی بدن نیز کمترین میزان پروتئین در تیمار 3 بود اما در میزان پروتئین، چربی، خاکستر و رطوبت بین شاهد و سایر تیمارها اختلاف معنیداری مشاهده نشد (P>0.05). بهطور کلی پروتئین هیدرولیز شده کانولا میتواند عملکرد رشد را در فیل ماهی بهشکل مثبتی تحت تاثیر قرار دهد. | ||
| کلیدواژهها | ||
| فیل ماهی؛ پروتئین هیدرولیز شده؛ بیان ژن و ترکیب شیمیایی بدن | ||
| عنوان مقاله [English] | ||
| The effects of hydrolyzed canola meal protein on growth, body composition and expression of growth genes and appetite of beluga fish (Huso huso) | ||
| نویسندگان [English] | ||
| Mahmoudreza Ebrahimnezhadarabi1؛ Seyed Mehdi Hosseinifard2؛ Reza Changizi3؛ Saber Vatandoust4؛ Shayan Ghobadi5 | ||
| 1PhD Student in Fisheries, Department of Fisheries, Babol Branch, Islamic Azad University, Babol, Iran. | ||
| 2Assistant Professor, Department of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran. | ||
| 3Department of Aquaculture, Faculty of Veterinary, IAU, Babol branch | ||
| 4Assistant Professor, Department of Fisheries, Babol Branch, Islamic Azad University, Babol, Iran | ||
| 5Assistant ProfessorDepartment of Aquaculture, Islamic Azad University, Babol Branch | ||
| چکیده [English] | ||
| The aim of this study was to investigate the effects of increasing the hydrolyzed protein of canola meal on growth factors, expression of GH, IGF and ghrelin genes and beluga (Huso huso) juvenile body composition. For this purpose, 840 juveniles beluga with an average weight of 30±5grams were distributed in 12 tanks, 70 in each tank. Each tank has a capacity of 2400 liters. Canola meal hydrolyzed protein was fed with different levels of 0 (control), 300 (treatment 1), 400 (treatment 2) and 500 (treatment 3) mg of protein for 8 weeks. At the end of the breeding period, bioassay was performed to evaluate growth factors and biopsy of brain, stomach and liver tissue to evaluate the expression of these genes. The results showed that with increasing the amount of hydrolyzed protein in canola meal in the diet, body weight gain, specific growth rate and protein efficiency increased and the highest value was in treatment 3 (500 mg) which was a significant difference with other groups(P <0.05). Also, the expression of all 3 genes showed an increasing trend that the difference between treatments and control was significant (P <0.05). The highest expression was observed for all three genes in the 500 mg treatment. The lowest amount of protein was in treatment 3, but there was no significant difference in the amount of protein, fat, ash and moisture between the control and other treatments (P> 0.05). In general, hydrolyzed canola protein can positively affect growth performance in beluga fish. | ||
| کلیدواژهها [English] | ||
| beluga fish, hydrolyzed protein, gene expression and body chemical composition | ||
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| مراجع | ||
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