پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 25 |
| تعداد شماره‌ها | 938 |
| تعداد مقالات | 7,697 |
| تعداد مشاهده مقاله | 12,622,994 |
| تعداد دریافت فایل اصل مقاله | 8,986,011 |
اثر الیسیتورها و ترکیب فیتوهورمون‌ها بر رشد، محتویات رنگیزه‌های فتوسنتزی و پروتئین جلبک sorokiniana Chlorella | ||
| زیست شناسی کاربردی | ||
| مقاله 4، دوره 31، شماره 4 - شماره پیاپی 58، اسفند 1397، صفحه 47-60 اصل مقاله (256.82 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2019.4223 | ||
| نویسندگان | ||
| آمنه جمشیدی* 1؛ محمدعلی ابراهیمی2؛ طیبه رجبیان3؛ غلامرضا بخشی خانیکی4؛ شهلا مظفری5 | ||
| 1- مربی ، گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه پیام نور تهران، ایران | ||
| 2- دانشیار ، گروه کشاورزی، دانشکده کشاورزی، دانشگاه پیام نورتهران، ایران | ||
| 3دانشیار ، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه شاهد، تهران، ایران | ||
| 4استاد، گروه کشاورزی، دانشکده کشاورزی، دانشگاه پیام نورتهران، ایران | ||
| 5استادیار ، گروه شیمی، دانشکده علوم پایه، دانشگاه پیام نورتهران، ایران | ||
| چکیده | ||
| جلبک کلرلا کاربرد دارویی، صنعتی وغذایی دارد. به همین دلیل بالا بردن بیوماس ومتابولیت‌های آن ارزشمند است. هدف این پژوهش نیز به دست آوردن بهترین ماده یا ترکیب هورمونی برای افزایش بیوماس ومتابولیت‌هایارزشمند Chlorella sorokiniana بود. در این پژوهشجلبک C. sorokiniana در محیط کشت پایۀBold ((BBM اصلاح‌شدهکشت شد. به محیط‌ها نسبت‌های مختلف هورمونی نفتالن استیک اسید ((NAA، ایندول بوتیریک اسید ( (IBAوکینتین Kin) )، نیترات و فسفات و تیامین پیروفسفات افزوده شد. در تیمار با NAA (10 میلی‌گرم در لیتر) Kin+(یک میلی‌گرم در لیتر) وزن خشک، محتویات رنگیزه‌های فتوسنتزی و پروتئین نسبت به شاهد افزایش معنی‌داری یافت. این صفات در تیمار با IBA+Kin وتیامین پیروفسفات اختلاف معنی‌داری را با شاهد نشان نمی‌داد بالاترین افزایش معنی دار وزن خشک، محتویات رنگیزه‌های فتوسنتزی و پروتئین نسبت به شاهد در تیمار افزایش نیترات و فسفات مشاهده شد. | ||
| کلیدواژه‌ها | ||
| بیوماس؛ جلبک؛ نیترات وفسفات؛ هورمون گیاهی | ||
| عنوان مقاله [English] | ||
| Effects of elicitors and combined phytohormones on growth , photosynthetic pigments and protein contents of Chlorella sorokiniana | ||
| نویسندگان [English] | ||
| Amaneh Jamshidi1؛ MohamadAli Ebrahimi2؛ Tayebeh Rajabiyan3؛ GhoulamReza Bakhshi Khaniki4؛ Shahla Mouzafari5 | ||
| 1- Instructor, Department of Biology, Faculty of Basic Sciences, Payame Noor University, Tehran, Iran | ||
| 2- Associate Professor, Department of Agriculture, Faculty of Agriculture, Payam Nourtahan University, Iran | ||
| 3Associate Professor, Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran | ||
| 4Professor, Department of Agriculture, Faculty of Agriculture, Payame Nourtahan University, Iran | ||
| 5Assistant Professor, Department of Chemistry, Faculty of Basic Sciences, Payam Nourtahan University, Iran | ||
| چکیده [English] | ||
| Chlorella has applications in medicine, industry and food. For this reason, increase of biomass and its metabolites is valuable. Aim of this study was to obtain the best substance or phytohormone combinations to increase biomass and metabolites contents of Chlorella sorokiniana. In this study, Chlorella sorokiniana was cultured in modified Bold Basal Medium (BBM). Different proportions of plant hormones, naphthalene acetic acid (NAA), indolebutyric acid (IBA) and kinetin (Kin), nitrate and phosphate and thiamine pyrophosphate were added to medium. There was significant rise in dry weight, photosynthetic pigments and protein contents in treatment NAA (10mg.L-1) + Kin (1mg.L-1) compared with control. In treatment of IBA+ Kin, and thiamine pyrophosphate no significant difference was observed in these parameters. The highest significant enhancement of dry weight, metabolites and protein contents were observed in medium supplemented with higher amount of nitrate and phosphate. | ||
| کلیدواژه‌ها [English] | ||
| alga, biomass, nitrate and phosphate, plant hormone | ||
| مراجع | ||
|
Agrawal, S.S. and Paridhavi, M. (2007) Herbal Drug Technology. Universities Press, 730Pp, Hyderabad. Andersen, R. A. (2005) Algal Culturing Techniques. Elsevier Inc, 578Pp, New York. Arora, N., Patel, A., Pruthi, P. A. and Pruthi, V.(2016) Synergistic dynamics of nitrogen and phosphorous influences lipid productivity in Chlorella minutissima for biodiesel production. Bioresource Technology 213 : 79–87. Bajguz, A. and Piotrowska-Niczyporuk, A. (2013) Synergistic effect of auxins and brassinosteroids on the growth and regulation of metabolite content in the green alga Chlorella vulgaris (Trebouxiophyceae). Plant Physiology and Biochemistry 71:290-7. Bajguz, A. (2000) Effect of brassinosteroids on nNucleic acids and protein content in cultured of Chlorella vulgaris. Plant Physiology and Biochemistry 38: 209–215. Bajguz, A. and Piotrowska-Niczyporuk, A. (2014) Interactive effect of brassinosteroids and cytokinins on growth, chlorophyll, mono saccharide, and protein content in the green alga Chlorella vulgaris (Trebouxiophyceae). Plant Physiology and Biochemistry 80:176-83. Czerpak, R., Bajguz, A., Białecka, Ba., Wierzchołowska, L. E. and Wolańska , M. M. (1994)Effect of auxin precursors and chemical analogues on the growth and chemical composition in Chlorella pyrenoidosa, Acta Societatis Botanicurm Poloniae 63: 279-286. Fried, S., Mackie, B. and Nothwehr, E. (2003) Nitrate and phosphate levels positively affect the growth of algae species found in Perry Pond. Tillers 4: 21-24. Fu, L., Wei-feng, L. and Xiang-gen, SH. (2006) The Effects of NAA on the growth, chlorophyll and protein content of Chlorella vulgaris. Journal of Yancheng Institute of Technology 2: 917-921. García-Jiménez, P., Rodrigo, M. and Robaina, R.R. (1998) Influence of Plant growth regulators, polyamines and glycerol interaction on growth and morphogenesis of carposporelingsof Grateloupia doryphora cultured in vitro. Journal of Applied Phycology 10 :95–100. Hunt, R.W., Chinnasamy S., Bhatnagar, A. and Das, K.C. (2010) Effect of biochemical stimulants on biomass productivity and metabolite content of the microalgae, Chlorella sorokiniana. Applied Biochemistry and Biotechnology 162: 2400-2410. Hunt, R.W., Chinnasamy, S. and Das, K.C. (2011) The effect of naphthalene-acetic acid on biomass productivity and chlorophyll content of green algae, coccolithophore, diatom, and cyanobacterium cultures. Applied Biochemistry and Biotechnology164:1350–1365. Kawano, T. (2003) Roles of reactive oxygen species-generating peroxidase reactions in plant defense and growth induction. Plant Cell Reports. 9:829–837. Kiseleva, A.A., Tarachovskaya, E.R. and Shishova, M.F. (2012) Biosynthesis of phytohormones in algae. Russian Journal of Plant Physiology 59: 595-610. Kobayashi, N., Noel, E., Barnes, A., Watson, A., Rosenberg, J., Erickson, G. and Oyler, G. (2013) Characterization on three Chlorella sorokiniana strains in anaerobic digested effluent from cattle manure. Bioresource Technology 150: 377–386. Li, Z., Yuan, H., Yang, J.Sh. and Li, B.Z. (2011) Optimization of the biomass production of oil algae Chlorella minutissima UTEX2341. Bioresource Technology 102 9128–9134. Lu, Y. and Xu, J. (2015) Phytohormones in microalgae: a new opportunity for microalgal biotechnology. Trends in Plant Science 20: 273-282. Lui, J., Qiu, W., Song, Y., Peng, H. and Zhao, Y. (2017a) The growth and lipid productivity of Chlorella pyrenoidosa enhanced by plant hormones under ammonium stress. Environmental Progress & Sustainable Energy 0 : 1-7. Lui, T., Liu, F., Wang, C., Wang, Z. and Li, Y. (2017b) Lipid accumulation in Chlorella vulgaris by supplementation of synthetic phytohormone analogs. Bioresource Technology 232: 44-52. Mehdizadeh Allaf, M. (2013) Effect of plant hormones on the production of biomass and lipid in microalgae. Thesis (Master of Engineering Science ) University of Western Ontario London, Ontario Canada. Pp109. Nigam, S., Prakash Rai, M. and Sharma, R. (2011) Effect of nitrogen on growth and lipid content of Chlorella pyrenoidosa. American Journal of Biochemistry and Biotechnology 7 : 124-129. Ozioko, F.U., Chiejina, N.V. and Ogbonna, J.C. (2015) Effect of some phytohormones on growth characteristics of Chlorella sorokiniana IAM-C212 under photoautotrophic conditions. African Jounal of Biotechnology 14: 2367-2376. Piotrowska-Niczyporuk, A. and Bajguz, A. (2014) The effect of natural and synthetic auxins on the growth, metabolite content and antioxidant response of green alga Chlorella vulgaris (Trebouxiophycea). Plant Growth Regulator 73:57–66. Ramanna, L., Guldhe, A., Rawat, I. and Bux, F. (2014) The optimization of biomass and lipid yields of Chlorella sorokiniana when using wastewater supplemented with different nitrogen sources. Bioresource Technology 168:127-131. Ribeiro, A., Tesima, K., Souza, J. and Yokoya, N. (2013) Effects of nitrogen and phosphorus availabilities on growth, pigment, and protein contents in Hypnea cervicornis J. Agardh (Gigartinales, Rhodophyta). Journal of Applied Phycology 25: 1151-1157. Robles-Heredia, J.C., Sacramento-Rivero, J.C., Canedo-López, Y., Ruiz-Marín, A. and Vilchiz-Bravo, L. E.(2015) Amulti stage gradual nitrogen reduction strategy for increased lipid productivity and nitrogen removal in wastewater using Chlorella vulgaris and Scenedesmus obliquus. Brazilian Journal of Chemical Engineering 32: 335 – 345. Ruiz, J., Alvarez, P., Arbib, Z., Garrido, C., Barrag´an, J. and Perales, J.A. (2011) Effect of nitrogen and phosphorus concentration on their removal kinetic treated urban wastewater by Chlorella vulgaris. International Journal of Phytoremediation 13:884-96. Sharma, R., Singh, G.P. and Sharma, V.K. (2012) Effects of culture conditions on growth and biochemical profile of Chlorella vulgaris. Journal of Plant Pathology and Microbiology 3: 1000131. Srivasatava, L.M. (2002) Plant growth and development: hormones and environment. Academic Press. 772 pp, San Diego. Stirk, W.A., Bálint, P., Tarkowská, D., Novák, O., Strnad, M., Ördög, V. and Van Staden, J. (2013) Hormone profiles in microalgae: gibberellins and brasinosteroids. Plant Physioloy and Biochemistry 70:348-53. Stirk, W.A.,Bálint, P.,Tarkowská, D.,Novák, O., Maróti, G.,Ljung, K., Turečková, V.,Strand, M.,Ordög, V. and Staden, J. (2014) Effect of light on growth and endogenous hormones in Chlorella minutissima (Trebouxiophyceae). Plant Physioloy and Biochemistry 79: 66-76. Szechyn ´ska-Hebda, M., Skrzypek, E., Da˛browska, G., Biesaga-Kos ´cielniak, J., Filek, M. and Wedzony, M. (2007) The role of oxidative stress induced by growth regulators in the regeneration process of wheat. Acta Physiologia Plantarum.29:327–33. Tarakhovskaya, E.R.,. Maslov, Y.I. and Shishova, M. (2007) Phytohormones in algae. Russian Journal of Plant Physiology 54:163-170. Tate, J.J., Gutierrez-Wing, M.T., Rusch, K.A. and Benton, M.G. (2012) The effects of plant growth substances and mixed cultures on growth and metabolite production of green algae Chlorella sp.: A Review. Journal of Plant Growth Regulation 32:417–428. Wellburn, A.R. (1994) The spectral determination chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of Plant Physiology 144: 307-313. Yokoya, N.S. and Handro, W. (1996) Effects of auxins and cytokinins on tissue culture of Grateloupia dichotoma (Gigartinales, Rhodophyta). Hydrobiology 326/327: 393–400. Zabochnicka-Swiatek, M. (2010) Algae feedstock of the future. Archivum Combustionis 30 : 225–236. | ||
|
آمار تعداد مشاهده مقاله: 440 تعداد دریافت فایل اصل مقاله: 456 |
||