پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 25 |
| تعداد شماره‌ها | 916 |
| تعداد مقالات | 7,522 |
| تعداد مشاهده مقاله | 12,232,545 |
| تعداد دریافت فایل اصل مقاله | 8,651,834 |
بهینه‌سازی تولید آنزیم آلکالین پروتئاز به سویه‌ای از جنس Bacillus جدا شده از خاک | ||
| زیست شناسی کاربردی | ||
| مقاله 3، دوره 23، شماره 2، بهمن 1389، صفحه 23-29 اصل مقاله (184.32 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2011.3293 | ||
| نویسنده | ||
| معصومه انوری | ||
| . استادیار گروه میکروبیولوژی دانشگاه آزاد اسلامی واحد رشت | ||
| چکیده | ||
| 22 سویه باکتری قلیادوست از خاک‌های نمکی قلیایی استان گیلان در محیط milk Agar به‌منظور بررسی توانایی تولید آنزیم آلکالین پروتئاز جداسازی شد. از میان باکتری‌های جداسازی شده، اعضای جنس باسیلوس بیشترین توانایی تولید را نشان دادند. بررسی اثر درجه‌ی حرارت، زمان انکوباسیون، منبع کربن، ازت و pH نشان داد که بهترین شرایط تولید، به‌ترتیب در C ْ40 به‌مدت 20 ساعت با استفاده از 5/1% گلوکز (وزنی به حجمی)، 1% سولفات آمونیوم (وزنی به حجمی) و 5/9 = pH است | ||
| کلیدواژه‌ها | ||
| آلکالین پروتئاز؛ گونه‌ی باسیلوس؛ قلیادوست | ||
| عنوان مقاله [English] | ||
| Production and optimization of alkaline protease from Bacillus species isolated from soil | ||
| نویسندگان [English] | ||
| Maasoumeh Anvari | ||
| Department of Microbiology, Islamic Azad University, branch of Rasht, Rasht, Iran | ||
| چکیده [English] | ||
| 22 strain of alkalophilic bacteria isolated from Guilan alkali soils were screened on milk agar medium for their ability to produce alkaline protease. Among isolated bacteria, bacillus spp. was show the highest enzyme production ability. Maximum enzyme production was achieved with sugar 1.5% w/v (as sole carbon source), ammonium sulfate 1% (as sole nitrogen source), pH= 9.5 and 40˚C over a 24 hrs. incubation | ||
| کلیدواژه‌ها [English] | ||
| alkaline protease, Bacillus spp, alkalophilic | ||
| مراجع | ||
|
Altschul S.F., Madden T.L., Scha-Ver A.A., Zhang J., Zhang Z., Miller W., Lipman D.J.; (1997). “Gapped BLAST and PSI-BLAST: a new generation of protein database search programs”. Nucleic Acids Research. 25: 3389–3402. Chi Z., Ma C., Wang P., Li H.F.; (2007). “Optimization of medium and cultivation conditions for alkaline protease production by the marine yeast Aureobasidium pullulans” Bioresource Technology. 98: 534–538. Chu I.M., Lee C., Li T.S.; (1992). “Production and degradation of alkaline protease in batch cultures of Bacillus subtilis ATCC 14416” Enzyme Microbial and Technology. 4: 55–61. Dutta J.R., Dutta P.K., Banerjee R., (2004). “Optimization of culture parameters for extracellular protease production from a newly isolated Pseudomonas sp. using response surface and artificial neural network models” Process Biochemistry. 39: 2193–2198.
Elibol M., Moreira A.R.; (2005). “Optimizing some factors affecting alkaline protease production by a marine bacterium Teredinobacter turnirae under solid substrate fermentation” Process Biochemistry. 40: 1951–1956. Fujiwara N., Yamamoto K., Masui A.; (1991). “Utilization of a thermostable alkaline protease from an alkalophilic thermophile for the recovery of silver from used X-ray film” Journal of Fermentation and Bioengineering. 72: 306-308. Ghorbel S., Souissi N., Triki-Ellouz Y., Dufosse L., Guérard F., Nasri M.; (2005). “Preparation and testing of sardinella protein hydrolysates as nitrogen source for extracellular lipase production by Rhizopus oryzae” World Journal of Microbioligy and Biotechnology. 21: 33–38. Gupta R., Beg Q.K., Khan S., Chauhan B.;(2002). “An overview on ferme-ntation downstream processing and properties of microbial alkaline proteases”, Applied Microbiology and Biotechnology. 60: 381–395. Haddar A., Fakhfakh-Zouari N., Hmidet N., Frikha F., Nasri M., Kamoun A.S.; (2010). “Low-cost fermentation medium for alkaline protease produ-ction by Bacillus mojavensis A21 using hulled grain of wheat and sardinella peptone”, Journal of Bioscience and Bioengineering. 110: 288–294. Haddar A., Bougatef A., Agrebi R., Sellami-Kamoun A., Nasri M.; (2009). “A novel surfactant-stable alkaline serine-protease from a newly isolated Bacillus mojavensis A21. Purification and characterization”, Process Biochemistry. 44: 29–35. Hanlon G.W., Hodges N.A., Russel, A.D.; (1982). “The influence of glucose ammonium and magnesium availability on the production of protease and bacitracin by Bacillus licheniformis” ,Journal of General Microbiology. 128: 845–851. Johnvesly, B. Naik G.R.; (2001). “Studies on production of thermo-stable alkaline protease from therm-ophilic and alkaliphilic Bacillus sp. JB-99 in a chemically defined medium”, Process Biochemistry. 37: 139–144. Kirk O., Borchert T. V., Fuglsang C. C.; (2002). “Industrial enzyme applic-ations”. Current Opining Biotech-nology. 13: 345–351. Mukherjee A.K., Adhikari H., Rai S.K.; (2008). “Production of alkaline protease by a thermophilic Bacillus subtilis under solid-state fermentation (SSF) condition using Imperata cylindrica grass and potato peel as low-cost medium: characterization and application of enzyme in deter-gent formulation” Biochemical Engin-eering Journal. 39: 353–361. Potumarthi R., Subhakar C., Jetty A.; (2007). “Alkaline protease production by submerged fermentation in stirred tank reactor using Bacillus licheni-formis NCIM-2042: Effect of aeration and agitation regimes”, Biochemical Engineering Journal. 34: 185–192. Rai S. K., Mukherjee A.K.; (2009). “Ecological significance and some biotechnological application of an organic -solvent stable alkaline serine protease from Bacillus subtilis strain DM-04” Bioresour Technology. 100: 2642–2645. Sen S., Satyanarayana T.; (1993). “Optimization of alkaline protease production by thermophilic Bacillus licheniformis S-40”. Industerial. Jour-nal of Microbiology. 33: 43-47. Singh J., Vohra R.M., Sahoo D.K.; (2004). “Enhanced production of alkaline proteases by Bacillus sphaericus using fed-batch culture”, Process Biochemistry. 39: 1093–1101. Sundararajan S., Kannan N., Chittibabu S.; (2011). “Alkaline protease from Bacillus cereus VITSN04: Potential application as a dehairing agent” ,Journal of Bioscience and Bioen-gineering 111: 128–133. Triki-Ellouz Y., Ghorbel B., Souissi N., Kammoun S., Nasri M.; (2003). “Biosynthesis of protease by Pseud-omonas aeruginosa MN7 grown on fish substrate”, World Journal of Microbioligy and Biotechnology. 19: 41–45. Uyar F., Baysal Z.; (2004). “Production and optimization of process param-eters for alkaline protease production by a newly isolated Bacillus sp. under solid state fermentation”, Process Biochemistry. 39: 1893–1898. Wang Q., Hou Y., Xu Z., Miao J., Li, G., (2007). “Optimization of cold-active protease production by the psychr-ophilic bacterium Colwellia sp. NJ341 with response surface methodology”, Bioresource Techno-logy. 99: 1926–1931. Ward O.P.; (1986). Proteolytic enzymes. Pp. 789-815. in:M. Moo-Young (eds). Comprehensive Biotechnology, vol. III, Academic Press, New York. Yanga J.K., Shihb I.L., Tzengc Y.M., Wanga S.L.; (2000). “Production and purification of protease from a Bac-illus subtilis that can deproteinize crustacean wastes” Enzyme and Microbial Technology 26: 406–413.
| ||
|
آمار تعداد مشاهده مقاله: 483 تعداد دریافت فایل اصل مقاله: 290 |
||