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اثر تنش یخ زدگی بر برخی پاسخ‌های فیزیولوژیکی و آنتی‌اکسیدانی گل‌های زینتی میمونی (Antirrhinum majus) و بنفشه (Viola × wittrockiana) | ||
| زیست شناسی کاربردی | ||
| مقاله 5، دوره 36، شماره 2 - شماره پیاپی 76، شهریور 1402، صفحه 81-94 اصل مقاله (1.42 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2023.41369.1504 | ||
| نویسندگان | ||
| بهروز صالحی اسکندری* 1؛ زهره نصیریان جزی2؛ جلیل عباس‌پور3 | ||
| 1استادیار، گروه زیست‌شناسی، دانشگاه پیام نور، تهران، ایران | ||
| 2دانش‌آموخته ارشد بیوشیمی، گروه زیست‌شناسی، دانشگاه پیام نور، تهران، ایران | ||
| 3دانش‌آموخته دکتری فیزیولوژی گیاهی دانشگاه اصفهان | ||
| چکیده | ||
| مقدمه: یخ زدگی یکی از تنش‌های غیرزیستی است که اثرات زیانباری بر رشد و بهره‌وری گیاهان دارد. این پژوهش به منظور بررسی برخی پاسخ‌های فیزیولوژیکی و بیوشیمیایی دو گیاه زینتی و مقاوم به سرما شامل گل میمونی (Antirrhinum majus) و بنفشه (Viola × wittrockiana) در شرایط تنش یخ‌‌زدگی انجام شد. مواد و روش‌ها: از کمینه دمایی دی‌ماه سه مکان مختلف گلخانه، شهر اصفهان و فریدون‌شهر (به ترتیب دماهای 20، 3- و 11- درجه سانتی‌گراد) جهت اعمال تیمارهای مختلف دمایی بر روی گیاهان 70 روزه کشت شده در گلدان به‌مدت 15روز استفاده شد. نتایج: اگرچه روند تغییرات نسبت کلروفیل a و b در دو گیاه متفاوت بود، اما با افزایش برودت هوا رشد طولی ساقه روند کاهشی داشت بطوری‌که در پایین‌ترین دما، طول ساقه گل میمونی و بنفشه به‌ترتیب 63 و 50 درصد نسبت به گیاهان رشد یافته در دمای 20 درجه سانتی‌گراد کاهش داشت. مقدار ترکیبات فنلی، کربوهیدرات‌های محلول و هیدروژن‌پراکسید روند افزایشی داشت. همچنین افزایش قابل توجهی در فعالیت آنزیم کاتالاز هر دو گیاه و آسکوربات پراکسیداز گل میمونی تحت برودت 11- درجه سانتی‌گراد مشاهده گردید اما فعالیت آنزیم گایاکول پراکسیداز در هیچ یک از سطوح دمای انجماد تغییر معنی‌داری نداشت. بحث: بنابراین به نظر می‌رسد گیاه گل میمونی و بنفشه با بکارگیری سازوکارهای تنظیم اسمزی و با توانایی‌های متفاوت آنزیم‌های آنتی‌اکسیدان، می‌توانند در برابر تنش حاصل از انجماد مقاومت کنندکه نشان‌دهنده راهکارهای مقاومتی متفاوت وابسته به ژنوتیپ آنهاست. | ||
| کلیدواژه‌ها | ||
| ترکیبات فعال اسمزی؛ تنش سرما؛ رنگدانه‌های فتوسنتزی؛ گیاهان زینتی | ||
| عنوان مقاله [English] | ||
| Effect of freezing stress on some physiological and enzymatic responses of Ornamental plant, viola (Viola × wittrockiana) and snapdragon (Antirrhinum majus) | ||
| نویسندگان [English] | ||
| Behrooz Salehi-Eskandari1؛ Zohre Nasirian Jazi2؛ Jalil Abbaspour3 | ||
| 1Assistant Professor, Department of Biology, Payame Noor University (PNU), Tehran, Iran | ||
| 2Graduated from MSc in biochemistry, Department of Biology, Payame Noor University (PNU), Tehran, Iran | ||
| 3Graduated from PhD in Plant Physiology, University of Isfahan | ||
| چکیده [English] | ||
| Introduction: Freezing is one of the abiotic stresses that has harmful effects on plant growth and productivity. This study was performed to investigate some physiological and biochemical responses of two ornamental and cold-resistant plants, including the viola (Viola × wittrockiana) and snapdragon (Antirrhinum majus) under freezing stress. Materials and methods: To apply different temperature treatments on 70-day-old plants, the minimum temperature in January was used in three different places, including the greenhouse, the city of Isfahan and Fereydunshahr (temperatures of 20, 3- and -11 ° C, respectively) for 15 days. Results: Changes in the chlorophyll a/b ratio were differences in the two plants with increasing freezing stress, but shoot length gradually decreased and at the lowest temperature in viola and snapdragon plants were 63 and 50% of their controls (20 °C), respectively. The content of phenolic compounds, soluble carbohydrates and hydrogen peroxide also increased. In addition, a significant increase in catalase activity was observed in both plants under freezing temperatures, while the increase in ascorbate peroxidase activity was significant only in snapdragon at -11 °C. However, no significant change in the activity of guaiacol peroxidase was found in two plants under freezing temperatures. Discussion: Therefore, it seems that snapdragon and violet plants can withstand freezing stress by using osmotic regulation mechanisms as well as different abilities of antioxidant enzymes, which indicate different resistance strategies depending on their genotype. | ||
| کلیدواژه‌ها [English] | ||
| Cold stress, Ornamental plants, Osmoticum, Photosynthesis pigments | ||
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