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غربالگری و جداسازی برخی از باکتری های تجزیه کننده ی فنول در خاک های حاوی آلاینده های نفتی | ||
| زیست شناسی کاربردی | ||
| مقاله 4، دوره 33، شماره 3 - شماره پیاپی 65، آذر 1399، صفحه 52-69 اصل مقاله (1.53 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2020.25979.1301 | ||
| نویسندگان | ||
| فائزه ربانی1؛ وهب جعفریان* 2؛ احمد آسوده3 | ||
| 1دانشجوی سابق کارشناسی ارشد،گروه زیست‌شناسی، دانشکده علوم، دانشگاه زنجان | ||
| 2دانشیار ، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه زنجان، ایران | ||
| 3استاد،گروه شیمی، دانشکده علوم، دانشگاه فردوسی مشهد | ||
| چکیده | ||
| پژوهش حاضر با هدف شناسایی ژنتیکی ژن 16S rDNA جدایه‌های برتر باکتری در حذف فنول به منظور زیست‌پالایی فنول انجام شد. دو جدایه برتر از نظر فعالیت بالای فنولازی گزینش و در محیط کشت با pH ها و دما، منابع کربنی و نیتروژنی مختلف بررسی شدند. جدایه اول در محیط کشت حاوی 3/. گرم در لیتر فنول به عنوان منبع کربن،2/.درصد پپتون و آمونیوم کلرید به عنوان منابع نیتروژن، 100 درصد فنول را پس از 24 ساعت در دمای 35 درجه سیلسیوس و 7 = pH حذف کرد و جدایه دوم در محیط کشت حاوی یک درصد ساکارز، 3/.گرم بر لیتر فنول به عنوان منبع کربن و 2/.درصد عصاره مخمر آمونیوم‌کلرید به عنوان منبع نیتروژن، 100 درصد فنول را پس از 24 ساعت در دمای 30 درجه سیلسیوس و 7 = pH حذف کرد. نتایج تعیین توالی و همردیفی توالی 16S rDNA نشان داد که جدایه اول باکتری Aneurinibacillus migulanus و جدایه دوم باکتری Paenarthrobacter nitroguajacolicusاست. دو جدایه‌ی گزینش‌شده در این پژوهش می‌توانند به عنوان گزینه‌های مناسبی در اهداف زیست‌پالایی فنول مورد توجه قرار گیرند. | ||
| کلیدواژه‌ها | ||
| Aneurinibacillus migulanus؛ Paenarthrobacter nitroguajacolicus؛ تجزیه فنول؛ زیست پالایی | ||
| عنوان مقاله [English] | ||
| Screening and isolation of some phenol-degrading bacteria from petroleum-contaminated soils | ||
| نویسندگان [English] | ||
| Faeze Rabbani1؛ Vahab Jafarian2؛ Ahmad Assodeh3 | ||
| 1MSC.Department of Biology, Faculty of Sciences, University of Zanjan, Zanjan, Iran | ||
| 2Associate Professor.Department of Biology, Faculty of Sciences, University of Zanjan, Zanjan, Iran | ||
| 3Professor.Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| چکیده [English] | ||
| This study was conducted to identify 16S rDNA in the superior phenol-degrading bacteria for phenol bioremediation purposes. Two strains were selected as superior ones with higher phenolase activity and investigated in culture media with different pHs, temperatures, and carbon and nitrogen sources. The first strain removed 100% phenol in the medium contained 0.3 g/L phenol as carbon source, 0.2 % peptone and ammonium chloride as nitrogen source after 24 h, at 35 ºC in pH 7. While the second strain removed 100% phenol in the medium contained 1 % sucrose, 0.3 g/L phenol as carbon source, 0.2 % yeast extract and ammonium chloride as nitrogen source after 24 h, at 30 ºC in pH 7. The results of sequencing and alignment of 16S rDNA sequence revealed that the first strain belongs to Aneurinibacillus migulanus and the second one to Paenarthrobacter nitroguajacolicus. Overall, the two selected strains from the research could be considered as an appropriate candidate in phenol bioremediation purposes. | ||
| کلیدواژه‌ها [English] | ||
| Aneurinibacillus migulanus, Bioremediation, Paenarthrobacter nitroguajacolicus, Phenol degradation | ||
| مراجع | ||
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