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بررسی تأثیر عوامل اکولوژیکی و ادافیکی برمواد مؤثره ی گیاه فرفیون (Euphorbia helioscopia L.) | ||
| زیست شناسی کاربردی | ||
| مقاله 1، دوره 33، شماره 3 - شماره پیاپی 65، آذر 1399، صفحه 9-24 اصل مقاله (1 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22051/jab.2020.5196 | ||
| نویسندگان | ||
| ناصر جعفری* 1؛ سیده مبارکه حسینی2؛ محمد علی ابراهیم زاده3 | ||
| 1دانشیار گروه زیست شناسی دانشگاه مازندران، ایران | ||
| 2دانشجوی کارشناسی ارشد سیتماتیک دانشگاه مازندران،ایران | ||
| 3استاد گروه شیمی دارویی دانشگاه علوم پزشکی ساری، ایران | ||
| چکیده | ||
| گیاه فرفیون (Euphorbia helioscopia L.) یکی از گیاهان دارویی مهم بوده که در سراسر دنیا در طب سنتی به کار می رود. این تحقیق به منظور بررسی تأثیر عوامل اکولوژیکی و ادافیکی بر مواد مؤثره ی این گیاه صورت گرفت. برای این منظور گیاه فرفیون از 3 منطقه با ارتفاع متفاوت (1861، 949 و 236 متر) و با 3 تکرار جمع آوری گردید. سپس عصاره با استفاده از متانول اسیدی تهیه و میزان فنول آن بر اساس منحنی استاندارد گالیک اسید و فلاونوئید بر اساس منحنی کوئرستین بدست آمد. هم چنین فعالیت آنتی اکسیدانی به 3 روش بدام اندازی DPPH، نیتریک اکساید و قدرت احیاکنندگی بررسی شد. این مطالعه به وضوح نشان داده است که فعالیت زیستی عصاره به میزان فنول و فلاونوئید آن بستگی دارد. نتایج حاصله از این مطالعه نشان می دهد که گیاه فرفیون منطقه ی چالو، بهترین فعالیت آنتی اکسیدانی را در آزمون نیتریک اکساید و در بالاترین غلظت (800 میکروگرم بر میلی لیتر) نسبت به دو منطقه ی دیگر دارا می باشد. هم چنین نتایج نشان داد که توانایی عصاره های گیاه فرفیون در سه منطقه ی مورد بررسی در مهار رادیکال های آزاد نیتریک اکساید وابسته به غلظت است و با افزایش غلظت فعالیت ضد رادیکالی آن ها افزایش می یابد.نتایج این مطالعه نشان داد که گونه ی فرفیون، دارای محتوای فنول و فلاونوئید کل و فعالیت آنتی اکسیدانی نسبتآ بالایی است که می توان از آن به عنوان یک گونه ی گیاهی دارویی بهره برد. | ||
| کلیدواژهها | ||
| عوامل اکولوژیکی؛ فرفیون؛ قدرت احیاکنندگی؛ ترکیبات ثانوی؛ فعالیت آنتی اکسیدانی | ||
| عنوان مقاله [English] | ||
| The effect of ecological and edaphic factors on the active ingredients of Euphorbia (Euphorbia helioscopia L.) | ||
| نویسندگان [English] | ||
| Naser Jafari1؛ Sayed Mobarakeh Hossaini2؛ Mohamad Ali Ebrahimzadeh3 | ||
| 1Associate Professor, Department of Biology, University of Mazandaran, Iran | ||
| 2M.Sc. student of systematics, University of Mazandaran,.Iran | ||
| 3Professor, Department of Pharmaceutical Chemistry, Sari University of Medical Sciences, Iran | ||
| چکیده [English] | ||
| Euphorbia helioscopia L. is one of the important medicinal plants used in traditional medicine all over the world. This study was conducted to investigate the effect of ecological and edaphic factors on the active ingredients of this plant. For this purpose, the Euphorbia plant was collected from 3 different altitudes (1861, 949 and 236 m) with 3 replications. The extract was then prepared using acidic methanol and its phenol content was calculated according to the standard curve of gallic acid and flavonoid based on the quercetin curve. Its antioxidant activity was also investigated in 3 methods of DPPH, nitric oxide and reducing power. This study has clearly shown that the biological activity of the extract depends on the amount of phenol and its flavonoids. The results of this study show that Euphorbia plant in Challufe region has the best antioxidant activity in the nitric oxide test and at the highest concentration (800 μg/ml) compared to the other two regions. Our results also showed that the ability of the extracts of Euphorbia in the three studied areas to inhibit nitric oxide free radicals was concentration dependent and increased with increasing concentration of their anti-radical activity. The results of this study showed that the species of Euphorbia had a high total phenol and flavonoid content and antioxidant activity. Which can be used as a medicinal plant species. | ||
| کلیدواژهها [English] | ||
| Ecological factors, Euphorbia helioscopia, Reducing power, Secondary compounds, Antioxidant activity | ||
| مراجع | ||
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Al-Younis, N.K. and Abdullah, A.F. (2009) Isolation and antibacterial evaluation of plant extracts from some medicinal plants in kurdistan region.Journal Duhok University. 12(1): 250-255. Black, C.A. (1979) Methodes of soil analysis. American Society of Agronomy. 2: 1572-1771. Bouyoucos, G.J. (962) Hydrometer method improved for making particle size analysis of soils. Agronomy Journal. 54: 464-465. Chang, C., Yang, M., Wen, H. and Chern, J. (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric method. Journal Food Drug Analysis. 10: 178-182. Dow, A.I., Cline, T.A. and Homing, E.V. (1981) Salt tolerance studies on irrigated mint. Bulletin of Agricultural Research Center, Washington State University, Pullman. 906 pp. Ebrahimzadeh, M.A., Nabavi, S.F., Nabavi, S.M. and Eslami, B. (2010) Antihemolytic and antioxidant activities of Allium paradoxum. Central European Journal of Biology. 5(3): 338-345. Ebrahimzadeh, M.A., Nabavi, S.F., Nabavi, S.M. and Pourmorad, F. (2010) Nitric oxide radical scavenging potential of some Elburz medicinal plants. African Journal of Biotechnology. 9(32): 5212-5217. Fazli, R., Nazarnezhad, N., Ebrahimzadeh, M.A. and Zabihzadeh, M. (2013) Evaluation of the antioxidant capacities and total phenolic contents of beech and oak Barks. Armaghane Danesh: 18(2): 137-145. Ghasemi, K., Ghasemi, Y. and Ebrahimzadeh, M.A. (2009) Antioxidant activity, phenol and flavonoids contents of 13 Citrus species peels and tissues. Pakistan Journal Pharmaceutical Sciences. 22(3): 277-281. Grudwald, J. and Buttel, K. (1996) European phytotherapeutics market drugs made in germany. Journal of Drugs Made in Germany. 39: 6-11. Hemmati, K.H., Sharifani, M., Kalati, H. and Badiee, P. (2008) Flavonoid content of Hawthorn (Crataeguus monogyna) in Iran. International Horticultural Congress. 765: 287-290. Hertog, M.G., Feskens, E.J., Hollman, P.C., Katan, M.B. and Kromhout, D. (1993) Dietary antioxidants flavonoids and risk of coronary heart disease: the Zutphen Elderly study. Lancet. 342: 1007-1011. Hinneburg, I., Damien Dorma, H.J. and Hiltunen, R. (2006) Antioxidant activities of extracts from selected culinary herbs and spices. Food Chemistry. 97(1): 9-122. Jaakola, L. and Hohtola, A. (2010) Effect of latitude on flavonoid biosynthesis in plants. Plant Celland Environmental. 1239-1241. Jahanban, A.S., Mahmoodzadeh, A., Hasanzadeh, A., Heidavi, R. and Jamei, R. (2008) Antioxidants and antiradicals in almound hull and shell (Amygdalus communis L.) as a function of genotype. Food Chemistry. 115: 529-533. Jayaprakasha, G.K. and Tamil selvi, K.K. (2003) Antibacterial and antioxidant activities of grape (Vitis Vinifera) seed extracts. Food Research International. 36: 117-122. Josuttis, M., Carlen, C., Crespo, P., Nestby, R., Toldam-Andersen, T.B., Dietrich, H. and Kruger, E. (2012) Acomparison of bioactire compound of strawberry fruit from Europe affected by genotype and latitude. Berry Research. 2(2): 73-93. Keltawi, N.E. and Croteau, R. (1987) Salinity depression of growth and essential oil formation in spearmint and marjoram and its reversal by foliar applied cytokinin. Phytochemistry. 26: 1333-134. Khoshsokhan, F., Babalar, M., Poormeidani, A. and Fatahi, M.R. (2015) Antioxidant Activity, Total Phenolics and oil content of some Thymus kotschyanus and Thymus daenensis populations. Plant Production and Technology. 7:153-162. Nazari, S., Nazarnezhad, N. and Ebrahimzadeh, M.A. (2013) Evaluation of antioxidant properties and total phenolic and flavonoids content of Eucalyptus camaldulensis and Pinus sylvestris bark. Iranian journal of Wood and Paper Science Research. 28(3): 522-533. Olsen, S.R., Cole, C.V., Watanabe, F.S. and Dean, L.A. (1954) Estimation of available phosphorus in soils by extraction with sodium bicarbonate. USDA, Cire. 939, U. S. Gover. Prin. Office, Washington DC. Oncel, I., Yurdakulol, E., Keles, Y., Kurt, L. and Yildiz, A. (2004) Role of antioxidant defense system and biochemical adaptation on stress tolerance of high mountain and steppe plants. Acta Oecologica. 26: 211-218. Oomaha, B.D. and Mazza, G. (1996) Flavonoids and antioxidative activities in buckwheat. Journal of Agricultral and Food Chemistry. 44(7): 1746-1750. Ozturk, A., Hpek, A., Unlukara, A. and Gurbuz, B. (2004) Effects of salt stress and water deficit on plant growth and essential oil content of lemon balm depression of growth and essential oil formation in (Melissa officinalis L.). Pakistan Journal of Botany. 36: 782-792. Pieroni, A., Quave, C.L. and Santorio, R.F. (2004) Folk pharmaceutical knowledge in the territory of the Dolomiti Lucane, inland southern Italy. Journal of Ethnopharmacology. 95: 373-384. Rabiei, K.H., Bekhradnia, S., Nabavi, S.M., Nabavi, S.F. and Ebrahimzadeh, M.A. (2012) Antioxidant activity of polyphenol and ultrasonic extracts from fruits of Crataegus pentagyna subsp. elburensis. Natural Product Research. 26(24): 2353-2357. Rahbarian, R., Azizi, E., Behdad, A. and Mirbolook, A. (2019) Evaluation of Cr stress tolerance in Portulaca oleracea L. based on growth, photosynthesis indexs and the activity of antioxidant enzymes. Applied Biology. 32(1): 33-56. Rakic, S., Petrovic, S., Kukic, J., Jadranin, M., Tesevic, V. and Povrenovic, D. (2007) Influence of thermal treatment on phenolic compounds and antioxidant properties of oak acorns from Serbia. Food Chemistry. 104: 830-834. Shaghaghi, A. Nejad Ebrahimi, S. and Sonboli, A. (2019) Study of total phenolic, total flavonoid content and antioxidant potential in various organs of genus Papaver and Glaucium collected from Iran. Journal of Plant Production Research. 26(2): 195-214. Singleton, V.L. and Rossi, J.A. (1965) Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. American Journal of Enology and Viticulture. 16: 144-158. Stark, S., Julkunen tiitto, R., Holappa, E., Mikkola, K. and Nikula, A. (2008) Concentration of foliar quercetin in natural populations of white birch (Betula pubescens) increase with latitude. Chemical Ecology. 34: 1382- 1391. Vinatoru, M., Toma, M., Radu, O., Filip, P.I., Lazurca, D. and Nason, T.T. (1997) The use of ultrasound for the extraction of bioactive principle from plant materials. Ultrasonics Sonochemistry. 4(2): 135-139. Walkley, A. and Black, I.A. (1934) An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Science. 37:29-38. Young Kil, H., Seong, E.S., Ghimire, B.K., Chung Hllm Kwon, S.S. and Goh, E.J. (2009) Antioxidant and antimicrobial activities of Crude sorghum extract. Food Chemistry. 115: 1234-1239.
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